Michael A. Rudnicki

Phosphorylation of CARM1 at Ser572 preserves muscle stem cell self-renewal by restricting premature myogenic commitment

This study shows that phosphorylation of CARM1 at Ser572 is essential for maintaining muscle stem cell self-renewal by preventing premature myogenic commitment.

Regenerative Index reveals declining muscle regeneration in paediatric patients with Duchenne muscular dystrophy

The paper introduces the Regenerative Index (RI) to quantify muscle regeneration in DMD patients, finding a significant decline in RI with age.

Wnt7a is Required for Regeneration of Dystrophic Skeletal Muscle

This study shows that Wnt7a is critical for muscle regeneration in dystrophic mice, and systemic delivery of Wnt7a-containing extracellular vesicles can rescue regeneration deficits.

Wnt binding to Coatomer proteins directs secretion on exosomes independently of palmitoylation

This study identifies the structural mechanism by which Wnt7a binds to exosomes for secretion, showing it is independent of palmitoylation and involves a dilysine motif interacting with COPB2.

Isolation of small extracellular vesicles from regenerating muscle tissue using Tangential Flow Filtration and Size Exclusion Chromatography

The paper develops a protocol combining Tangential Flow Filtration and Size Exclusion Chromatography to isolate high-purity extracellular vesicles from muscle tissue, separating Wnt7a-bound EVs from free Wnt7a.

KDR Signaling in Muscle Stem Cells Promotes Asymmetric Division and Progenitor Generation for Efficient Regeneration

This study identifies KDR signaling as essential for muscle stem cell asymmetric division and progenitor generation, crucial for efficient muscle regeneration.