Shengnan Wang

Modeling CRISPR-Cas13d on-target and off-target effects using machine learning approaches

The paper develops DeepCas13, a deep learning model to predict CRISPR-Cas13d gRNA on-target activity and off-target viability effects, improving accuracy for RNA editing applications.

Repressor element 1-silencing transcription factor deficiency yields profound hearing loss through Kv7.4 channel upsurge in auditory neurons and hair cells

This study shows that REST deficiency leads to increased Kv7.4 channels in auditory neurons and hair cells, causing progressive hearing loss, which can be reversed by Kv7.4 inhibition.

Intrinsic RNA targeting constrains the utility of CRISPR-Cas13 systems

The paper evaluates CRISPR-Cas13 systems and finds that they have intrinsic RNA targets in mammalian cells, which can affect lentiviral particle production and limit their utility.