Mark W. Budde

Engineered protein circuits for cancer therapy

This paper engineers protease-based protein circuits that selectively kill RAS-mutant cancer cells and suppress tumors, showing resistance reduction and cytotoxicity independent of oncogene addiction.

Tuning methylation-dependent silencing dynamics by synthetic modulation of CpG density

The paper constructs promoters with varying CpG content to study how CpG density affects silencing rate via DNMT recruitment, finding a strong correlation and identifying a key CpG site.

Synthetic mammalian signaling circuits for robust cell population control

This paper designs a synthetic mammalian circuit using auxin for population control, showing it limits growth more robustly than non-paradoxical designs.

Imaging cell lineage with a synthetic digital recording system

A new genetic editing system termed intMEMOIR reveals the lineage histories of individual cells directly within their native tissue context.