Sue Biggins

The paper identifies an essential Ndc10 trimerization interaction in yeast kinetochores that stabilizes centromeric DNA and facilitates kinetochore assembly.

This study identifies an interdependent Cbf1-CCAN interaction that stabilizes the budding yeast kinetochore by coupling transcriptional roadblocking to inner kinetochore assembly.

The paper shows Mps1 phosphorylates MELT motifs in Stu1 to recruit Slk19 and mediate kinetochore clustering in budding yeast.

This study shows that Cnn1 stabilizes kinetochore-microtubule attachments by enabling Stu2-dependent reinforcement, as demonstrated through in vitro assays and genetic experiments.

The paper identifies 111 phosphorylation sites on KNL1, showing extensive phosphoregulation in response to microtubule-disrupting compounds.

This study directly observes the sequential and interdependent assembly of kinetochores on individual centromeres in budding yeast, revealing regulatory dependencies and a rate-limiting step in the process.

This study shows that K. marxianus has a single centromeric nucleosome that mediates attachment to one microtubule, similar to S. cerevisiae.

The paper uses Fiber-seq to show that regional centromeres have dichromatin organization, with accessible and compacted chromatin, which is conserved across species despite DNA sequence differences.

The paper investigates how the N-terminal domain of Cse4 stabilizes its association with centromeres in yeast, showing that OA and Scm3 independently stabilize Cse4 through their interactions with the END region.

This paper develops a computational model to predict DNA mechanics from sequence, revealing mechanical selection in diverse eukaryotes and identifying extreme DNA mechanics in nature.

The paper shows that kinetochores grip microtubules six-fold stronger when pulled toward plus ends, suggesting directional asymmetry in attachment strength contributes to mitotic accuracy.

This study reveals the architecture and flexibility of native kinetochores using structural analyses of purified kinetochores from thermophilic yeast.

This study identifies a Stu2-mediated tension-sensing pathway that works with Aurora B to correct kinetochore attachments in vivo.