This study identifies CxaR as an adaptor protein that facilitates ClpXP-mediated degradation of Sle1, regulating cell splitting in Staphylococcus aureus.

PLoS Genetics · Sep 5, 2025Peer-reviewedMicrobiology

Temperature-dependent regulation of bacterial cell division hydrolases by the coordinated action of a regulatory RNA and the ClpXP protease

Viktor Hundtofte Mebus, Larissa Milena Busch, Morten Børre, Tobias K. Nielsen, Martin S. Bojer, Camilla Henriksen, Maria Disen Barbuti, Danae Morales Angeles, Kamilla Ankær Brejndal, Stephan Michalik, Manuela Gesell Salazar, Morten Kjos, Uwe Völker, Birgitte Haahr Kallipolitis, Dorte Frees

This study identifies a temperature-sensitive regulatory mechanism in Staphylococcus aureus involving an RNA and ClpXP protease that controls cell division hydrolases.

The Cell Surface · May 15, 2025Peer-reviewedMicrobiology

The Spx stress regulator confers high-level β-lactam resistance and decreases susceptibility to last-line antibiotics in methicillin resistant Staphylococcus aureus

Tobias K. Nielsen, Ida Birkjær Petersen, Lijuan Xu, Maria Disen Barbuti, Viktor Hundtofte Mebus, Anni Justh, Abdulelah A. Alqarzaee, Nicolas Jacques, Cécile Oury, Vinai Chittezham Thomas, Morten Kjos, Camilla Henriksen, Dorte Frees

This study identifies Spx as a stress regulator that enhances β-lactam resistance and reduces susceptibility to last-line antibiotics in MRSA through a redox-independent mechanism.

Antimicrobial Agents and Chemotherapy · May 1, 2024Peer-reviewedMicrobiology