Jonathon Pines

Engineering and characterisation of AAVS1 FRT cell lines for inducible and dose-dependent gene expression

This paper develops a stable RPE1 cell line with inducible and dose-dependent transgene expression using CRISPR/Cas9 and FRT/Tet-On systems, validated through multi-omics analysis.

Why is Cyclin A degraded before Cyclin B?

This study shows that rapid degradation of CyclinA ensures proper chromosome congression and genomic stability by preventing aneuploidy and malformed nuclei.

Mechanistic basis for the spatiotemporal assembly of the Bloom Syndrome complex on ultrafine DNA bridges

This study identifies how the BTRR complex assembles on ultrafine DNA bridges through SLiM recognition by RMI1-RMI2, revealing spatiotemporal regulation during DNA repair and segregation.

Single kinetochores execute an ordered series of molecular events as the Spindle Assembly Checkpoint is silenced

The paper maps the ordered molecular events of SAC silencing at single kinetochores using MAD2 levels, revealing sequential unbinding, structural reorganization, and stabilization processes.

High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells

This study presents a proteomic and phosphoproteomic analysis of the human cell cycle in non-transformed cells, revealing new insights into cell cycle-dependent protein and phosphorylation oscillations.