Theodoros I. Roumeliotis

Why is Cyclin A degraded before Cyclin B?

This study shows that rapid degradation of CyclinA ensures proper chromosome congression and genomic stability by preventing aneuploidy and malformed nuclei.

Mechanistic basis for the spatiotemporal assembly of the Bloom Syndrome complex on ultrafine DNA bridges

This study identifies how the BTRR complex assembles on ultrafine DNA bridges through SLiM recognition by RMI1-RMI2, revealing spatiotemporal regulation during DNA repair and segregation.

BCOR mutations establish a persistent culture-adaptive state in human induced pluripotent stem cells

This study identifies BCOR mutations in hiPSCs as a culture-adaptive state causing chromatin, transcriptomic, and proteomic changes, and develops a qPCR assay for detection.

Structural basis of polyspecific drug recognition by MRP2

This study determines how MRP2 recognizes diverse drugs structurally, showing overlapping ligand binding with different interactions.

Cryo-EM structures of the CDK11-cyclin L-SAP30BP complex reveal mechanisms of CDK11 regulation

The paper presents the cryo-EM structure of the CDK11-cyclin L-SAP30BP complex, revealing mechanisms of CDK11 regulation and inhibitor selectivity.

CCDC103-mediated assembly of the R2C complex links RUVBL1-RUVBL2 to Primary Ciliary Dyskinesia

This study presents the cryo-EM structure of the R2C complex, revealing how CCDC103 interacts with RUVBL1-RUVBL2 to facilitate dynein motor assembly in PCD.

LOX inhibition disrupts a collagen-integrin-MYC axis as a translatable targeting strategy in invasive lobular carcinoma

LOX inhibition disrupts a collagen-integrin-MYC axis in ILC, halting tumor growth and metastasis with minimal toxicity, offering a new therapeutic strategy.

A redox switch in p21-CDK feedback during G2 phase controls the proliferation-cell cycle exit decision

The paper identifies a redox-sensitive cysteine in p21 that regulates its interaction with CDKs, controlling cell cycle progression and proliferation through a feedback loop.

MAGELLAN: Automated Generation of Interpretable Computational Models for Biological Reasoning

MAGELLAN constructs interpretable computational models from pathway data and experimental rules, demonstrating predictive accuracy in breast cancer and NSCLC signalling.

Chromosome end protection by RAP1-mediated inhibition of DNA-PK

The paper shows RAP1 inhibits DNA-PK's end joining function at telomeres by forming a complex with TRF2, preventing telomere fusions.

High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells

This study presents a proteomic and phosphoproteomic analysis of the human cell cycle in non-transformed cells, revealing new insights into cell cycle-dependent protein and phosphorylation oscillations.

PBRM1 directs PBAF to pericentromeres and protects centromere integrity

This study identifies PBAF as a protector of centromere and pericentromere structure, showing that PBRM1 loss leads to centromere fragility and therapeutic vulnerability.

Selection of pre-leukemic hematopoietic stem cells driven by distinct extracellular matrix molecules

The paper characterizes Tet2-mutant HSCs using multi-omic data and identifies ECM molecules as regulators of self-renewal in pre-leukemic HSCs.

Integrated multiomic profiling reveals SWI/SNF subunit-specific pathway alterations and targetable vulnerabilities

The paper identifies pathway alterations and targetable vulnerabilities in SWI/SNF-defective cancers through multiomic profiling and drug screening.

m6a methylation orchestrates IMP1 regulation of microtubules during human neuronal differentiation

The paper shows m6A methylation guides IMP1's regulation of microtubule-related mRNAs during human neuronal differentiation.

A rare genetic disorder provides insights into mechanisms of early-onset neurodegeneration

This study investigates neurodegeneration in Xeroderma pigmentosum using stem cell-derived neurons, identifying endoplasmic reticulum stress and UPS dysfunction as key factors and developing an early detection assay.

Mechanism of assembly, activation and lysine selection by the SIN3B histone deacetylase complex

The paper presents the structure of the SIN3B histone deacetylase complex and elucidates its mechanism of substrate recognition and catalysis.

Characterization of proteome-size scaling by integrative omics reveals mechanisms of proliferation control in cancer

The paper uses multi-omic data to show how protein and mRNA scaling with cell size regulates proliferation in melanoma cells, revealing mechanisms of growth and division coupling.

Epithelial-Mesenchymal Plasticity is regulated by inflammatory signalling networks coupled to cell morphology.

The paper shows that epithelial-mesenchymal plasticity is regulated by inflammatory signalling networks linked to cell morphology through changes in microtubule and nuclear organization.

Dissecting mutational mechanisms underpinning signatures caused by replication errors and endogenous DNA damage

The paper identifies gene-specific mutational signatures caused by replication errors and endogenous DNA damage using CRISPR-Cas9 knockouts and whole-genome sequencing.

Multiplexed quantitative screens of single cell shape and YAP/TAZ localisation identify DOCK5 as a coincident detector of polarity and adhesion during migration.

The paper identifies DOCK5 as a regulator of YAP/TAZ localization and cell shape during migration, showing its role in maintaining polarity through interactions with CDC42, RAC, and RHOA.

Decoding the genomic basis of osteoarthritis

This study identifies molecular subgroups of osteoarthritis and develops a 7-gene classifier to stratify patients, while uncovering potential therapeutic compounds.

Evaluation of a Dual Isolation Width Acquisition (DIWA) method for isobaric labelling ratio decompression

The paper evaluates a DIWA method to decompress isobaric labelling ratios by using narrow isolation widths for quantification and standard widths for identification, improving accuracy while maintaining coverage.

A screen for combination therapies in BRAF/NRAS wild type melanoma identifies nilotinib plus MEK inhibitor as a synergistic combination

Integrative epigenomics, transcriptomics and proteomics of patient chondrocytes reveal genes and pathways involved in osteoarthritis

The paper identifies 49 genes and pathways associated with osteoarthritis progression using integrative epigenomics, transcriptomics, and proteomics in human chondrocytes from OA patients.

Genomic determinants of protein abundance variation in colorectal cancer cells

The paper quantifies proteomic and genomic variation in colorectal cancer cell lines to understand how mutations affect protein levels and drug responses.