The paper redesigns calmodulin to improve its function while preserving conformational dynamics, showing that structural flexibility is essential for physiological regulation.

bioRxiv · Apr 17, 2026 · PreprintBiophysics

Generation of human induced pluripotent stem cell (hiPSC) lines from patients with extreme high and low polygenic scores for QT interval

Devyn Mitchell, Rizwan Ullah, Loren R. Vanags, Alex Shen, Luke Jones, William Morris, Matthew J. O’Neill, Giovanni Davogustto, Christian M. Shaffer, Dan M. Roden, Ben B. Shoemaker, Hollie Williams, Teresa L. Strickland, Taylor A. Agee, Christopher N. Johnson, Brett M. Kroncke

The paper generates hiPSC lines from patients with extreme polygenic scores for QT interval to study LQTS genetic and cellular complexity.

Stem Cell Research · Feb 27, 2025Peer-reviewedGenetics

Prognostic Value of Multiplexed Assays of Variant Effect and Automated Patch-clamping for KCNH2-LQTS Risk Stratification

Matthew J. O’Neill, Chai‐Ann Ng, Takanori Aizawa, Luca Sala, Sahej Bains, Annika Winbo, Rizwan Ullah, Qianyi Shen, Chek-Ying Tan, Krystian A. Kozek, Loren R. Vanags, Devyn Mitchell, Alex Shen, Yuko Wada, Asami Kashiwa, , Brett M. Kroncke

This paper evaluates how functional variant data and penetrance estimates improve risk stratification for LQTS in KCNH2 missense variant carriers.

Circulation · Sep 24, 2024Peer-reviewedGenetic & Genomic Medicine

Environmentally stressed human nucleus pulposus cells trigger the onset of discogenic low back pain

Wensen Jiang, Juliane D. Glaeser, Giselle Kaneda, Julia Sheyn, Jacob T. Wechsler, Stephen R. Stephan, Khosrowdad Salehi, Julie Lynn Chan, Wafa Tawackoli, Pablo Avalos, Christopher N. Johnson, Chloe M. Castaneda, Linda E.A. Kanim, Teerachat Tanasansomboon, Joshua E. Burda, , Dmitriy Sheyn

The paper identifies a stress-induced subtype of nucleus pulposus cells in degenerating intervertebral discs that trigger discogenic low back pain.

bioRxiv · Mar 2, 2023 · PreprintMolecular Biology