The paper identifies dynamic acetylation changes in yeast during heat shock, showing acetylation regulates protein activity to enhance the heat shock response.

Genome biology · Jul 16, 2026Peer-reviewedGenomics

A new chemical carcinogen and Western diet protocol to reliably induce advanced hepatocellular carcinoma in male and female mice

Melina Cristina Mancini, Elise R Adams, David H. Burk, Richard P. Carmouche, Sara Webb, Jarosław Staszkiewicz, J. Michael Salbaum, Samuel G. Mackintosh, Timothy D. Heden

bioRxiv · Mar 10, 2026 · PreprintCancer Biology

YY1 relieves p300 autoinhibition to promote histone acetylation in advanced prostate cancer, enhancing the oncogenic signaling of Androgen Receptor Splice Variant 7

Chenxi Xu, Dongyu Wu, Bo Pan, Xiaoxiao Ren, Samuel G. Mackintosh, Kanishk Jain, Natarajan V. Bhanu, Ricky D. Edmondson, Xin Liu, Benjamin Aaron Garcia, Alan J. Tackett, Jian Jun Jin, Gang Greg Wang, Ling Cai

This study shows YY1 enhances p300 acetyltransferase activity in CRPC, promoting histone acetylation and oncogenic gene expression, and identifies a potential therapeutic target for overcoming drug resistance.

bioRxiv · Aug 4, 2025 · PreprintCancer Biology

Characterization of methionine dependence in melanoma cells

Sarita Garg, Lauren C. Morehead, Jordan T. Bird, Stefan Graw, Allen J. Gies, Aaron J. Storey, Alan J. Tackett, Rick D. Edmondson, Samuel G. Mackintosh, Stephanie D. Byrum, Isabelle Racine Miousse

This study investigates how melanoma cells respond to methionine restriction by comparing methionine-dependent and -independent cell lines, revealing differences in methylation, transcriptome, and proteome responses.

Molecular Omics · Aug 31, 2023Peer-reviewedMolecular Biology

Transection injury differentially alters the proteome of the human sural nerve

Monica J. Chau, Jorge E. Quintero, Eric M. Blalock, Stephanie D. Byrum, Samuel G. Mackintosh, Christopher Ala Samaan, Greg A. Gerhardt, Craig G. van Horne

This study identifies proteomic changes in human sural nerve after transection injury, revealing upregulated and downregulated proteins associated with regeneration and degeneration.

PLoS ONE · Nov 23, 2022Peer-reviewedNeuroscience

DNA-PKcs kinase activity stabilizes the transcription factor Egr1 in activated immune cells

Zachary J. Waldrip, Lyle J. Burdine, David K. Harrison, Ana Clara P. Azevedo‐Pouly, Aaron J. Storey, Olivia G. Moffett, Samuel G. Mackintosh, Marie Burdine

The paper identifies DNA-PKcs as a kinase that phosphorylates Egr1 at S301, stabilizing it and enhancing IL2 transcription in T cells during activation.

Journal of Biological Chemistry · Sep 22, 2021Peer-reviewedMolecular Biology

Accurate and Sensitive Quantitation of the Dynamic Heat Shock Proteome using Tandem Mass Tags

Aaron J. Storey, Rebecca E. Hardman-Kavanaugh, Stephanie D. Byrum, Samuel G. Mackintosh, Rick D. Edmondson, Wayne P. Wahls, Alan J. Tackett, Jeffrey A. Lewis

The paper quantifies the dynamic heat shock proteome in budding yeast using TMT-MS3, revealing differential protein expression and PTM regulation over time.

Journal of Proteome Research · Feb 6, 2020Peer-reviewedGenomics

Metaproteomics Reveals Potential Mechanisms by which Dietary Resistant Starch Supplementation Attenuates Chronic Kidney Disease Progression in Rats

Boris L. Zybailov, Galina V. Glazko, Yasir Rahmatallah, Dmitry S. Andreyev, Taylor McElroy, Oleg K. Karaduta, Stephanie D. Byrum, Lisa Dwyer Orr, Alan J. Tackett, Samuel G. Mackintosh, Ricky D. Edmondson, Dorothy A. Kieffer, Roy Joseph Martin, Sean H. Adams, Nosratola D. Vaziri, John M. Arthur

The paper uses metaproteomics to analyze cecum contents in CKD rats with and without resistant starch, revealing shifts in gut microbiota and host proteins associated with improved CKD outcomes.

PLoS ONE · Jan 30, 2019Peer-reviewedSystems Biology