Richard M. Murray

This paper implements a phosphorylation-based insulator in a cell-free system and identifies its structural parameters to reduce retroactivity.

The paper designs a pulsatile communication circuit in bacteria to enable dynamic patterning and long-distance communication through model-driven optimization of promoter and RBS strengths.

The paper describes a method to repress integrase activity at specific DNA sites using CRISPR-Cas9 in E. coli, achieving up to 8-fold repression of integration at identical attP sites.

This paper demonstrates the co-translational expression of active membrane proteins in a cell-free TX-TL platform using nanodiscs, enabling testing of complex biocircuits involving membrane-bound proteins.

This study shows that compositional context in synthetic gene networks significantly affects gene expression levels and key regulatory properties.

The paper develops a Bayesian method to infer chemical signal timing and amplitude from population-level response distributions in a temporal logic gate system in E. coli.

The paper investigates integrase dynamics in a cell-free system, finding that sequestration by binding sites dominates dynamics and reporter activation delay correlates with integrase concentration.

This paper discusses the development of E. coli-based cell-free systems for synthetic biology, focusing on standardizing extract and energy preparation methods.

The paper explores protein degradation in a TX-TL cell-free system using ClpXP protease, highlighting its role in circuit dynamics.

This study shows negative transcriptional feedback reduces temperature variation effects on circuit dynamics using mathematical models and experiments.

This paper characterizes a cell-free breadboard platform by measuring transcriptional and translational activities and analyzing resource limitations in gene circuit performance.

This study designs a temperature-robust incoherent feedforward loop circuit using control theory and experimental analysis.