Systematic optimization and benchmarking of synchro-PASEF for high-throughput phosphoproteome profiling
Dain R. Brademan, Angelina A. Mullarkey, Mia L. Greeson, Sarah Szvetecz, Olga Vitek A, Emily E. Blythe, Ruth Hüttenhain
This paper optimizes and benchmarks synchro-PASEF for phosphoproteomics, showing improved identification and quantification with retention time summation, and comparable performance to dia-PASEF in modeling phosphosignaling.
bioRxiv · Jun 27, 2026 · PreprintBiochemistry