Rajan Iyyappan

The paper develops a serum-free 6iL/E4 culture system that enables derivation and maintenance of embryonic stem cells (ESCs) across multiple mammalian species, revealing conserved mechanisms for ESC self-renewal.

This paper introduces CODEMax and CODEMax(exo+) genome editing systems that enable efficient and precise search-and-replace editing in human cells with reduced off-target effects.

This study investigates mRNA translation dynamics during mouse pre-implantation development, revealing novel mechanisms of translational selectivity and the role of Eif1ad3 in ribosome biogenesis.

This study uses SAC-seq to map m6A modifications at single-nucleotide resolution in bovine preimplantation embryos, identifying a critical m6A site in RPL12 that regulates translation and development.

The paper shows that DNA replication patterns in early mammalian embryos are established early, with late replicating regions near the nuclear lamina showing fragility and predisposing long genes to genetic changes.

The paper maps the single-cell transcriptomes of bovine peri-implantation development to identify cell lineages and molecular mechanisms involved in early pregnancy success and failure.

This study uses low-input ribosome profiling to analyze translational selectivity in bovine oocytes and preimplantation embryos, revealing four modes of translational regulation and their temporal dynamics.

This study identifies Tdrd3 as a regulator of meiosis II in mouse oocytes by controlling Emi2 mRNA translation, ensuring proper MII spindle formation.