Christoph W. Müller

This study visualizes human pre-ribosome assembly in nucleoli using cryogenic correlative light and electron tomography, revealing structural states and interactions.

EasyGrid automates cryo-EM sample preparation and quality control, improving vitrification for macromolecules and mammalian cells.

The paper investigates how Fpt1 is recruited to tRNA genes in budding yeast, revealing a dual role of TFIIIB and TFIIIC in its binding under different RNAPIII conditions.

This paper introduces a photoswitchable HaloTag (psHaloTag) that enables reversible fluorescence control in living cells using light.

The paper maps human RNA polymerase III transcription dynamics, finding that termination is the rate-limiting step for highly expressed tRNA genes.

The paper presents cryo-EM structures of the SNAPc-containing Pol III PIC on the U6 snRNA promoter and identifies structural differences in SNAPc engagement between Pol III and Pol II PICs.

This study uses cryo-EM and smFRET to reveal structural and kinetic details of yeast TFIIIC engagement with tRNA promoter DNA motifs.

This study presents cryo-EM structures of human TFIIIC complex bound to a tRNA gene, revealing how it recognizes promoter motifs and facilitates Pol III activation.

The paper investigates RNAPIII transcription termination in budding yeast, showing T-tracts are necessary but not sufficient, and secondary structures and Sen1 helicase are important for termination.

The paper determines the structure of UAF bound to DNA and TBP to reveal how UAF selects RNA Polymerase I for ribosome biogenesis and mediates specific protein-DNA interactions.

The paper reports high-resolution cryo-EM structures of human RNA polymerase I, revealing structural features and disease-related mutations.

This study identifies the PHF14 complex's role in DNA damage detection and repair through biomolecular condensate formation, showing its importance in neural progenitor cell function.

The paper presents cryo-EM structures of human RNA polymerase III in unbound and transcribing states, revealing structural insights into its function and disease associations.

The paper presents a cryo-EM structure of yeast Maf1 bound to Pol III, revealing how Maf1 represses transcription by sequestering initiation elements and blocking the active site.

The paper presents cryo-EM structures of yeast Pol I elongation complexes bound to GMPCPP, revealing structural rearrangements involving TFIIE/TFIIF-like and TFIIS-like subunits.

The paper introduces a synthetic biology approach to study nucleosome symmetry by creating asymmetric H3 heterodimers and demonstrates their utility in analyzing histone modification crosstalk.