Nicholas D. Leigh

Rag1-knockout newts lacking adaptive immunity regenerate larval limbs, tails and adult limbs without overt gross differences from controls; newt blastema tolerates allografts, and axolotl regenerating limbs show immunosuppressive transcriptional shifts.

Novabrowse enables high-resolution synteny analysis and ortholog detection by integrating BLAST results with genomic context, revealing gene presence/absence and chromosomal rearrangements in newt genomes.

This study shows Prdm16 enhances Notch signaling in arterial endothelial cells and suppresses venous fate to prevent arteriovenous malformations in zebrafish and human cells.

The paper presents the chromosome-scale assembly of the giant Pleurodeles waltl genome, revealing insights into its expansion and regeneration-related genes.

This paper demonstrates the use of O-PTIR for fast, non-destructive spatiotemporal chemical analysis of unprocessed biopsies, living brain tissue, and vertebrates.

This study validates SNP-based demultiplexing for pooled scRNA-seq data across multiple non-human species, showing accurate demultiplexing with only transcriptome data and no prior genetic reference.

This study identifies cell type innovations in salamander forebrain evolution, showing similarities to both sauropsid DVR and mammalian neocortex.

The paper presents a method for isolating high-quality RNA from human precision-cut lung slices, enabling RNA-seq analysis and computational integration with larger patient cohorts.

The paper identifies vwde expression as a conserved feature of blastemas involved in appendage regeneration across species and shows its functional requirement in regeneration.