Grant L. Hughes

Emergence Risks from Within? Metagenomic Analysis of Mosquito Viromes from Two Zoos Reveals Mosquito-associated Orthobunyaviruses in the UK

The paper analyzes mosquito viromes from UK zoos using metagenomics, identifying two novel Orthobunyaviruses, including one potentially arbovirus.

Vertical transmission of mosquito microbiota and its effects on offspring development

The paper investigates vertical transmission of microbiota in Aedes aegypti mosquitoes and finds that maternally transmitted bacteria influence offspring development and fitness.

Impact of thermal variation on the transcriptome of Plasmodium falciparum infected Anopheles stephensi

This study examines how temperature and time affect gene expression in Anopheles stephensi infected with Plasmodium falciparum, revealing coordinated transcriptional responses in midgut and systemic tissues.

Mosquito host background influences microbiome-ZIKV interactions in field and laboratory-reared Aedes aegypti

This study examines how mosquito genetic background and associated microbiomes influence Zika virus infection outcomes in Aedes aegypti, revealing line-specific microbiome responses to viral exposure.

Impact of temperature on vector competence of Culex pipiens molestus: implications for Usutu virus transmission in temperate regions

This study shows that Culex pipiens molestus mosquitoes can transmit Usutu virus at 22°C, with higher viral loads and transmission rates compared to lower temperatures.

SARS-CoV-2 Survival on Skin and its Transfer from Contaminated Surfaces

This study examines SARS-CoV-2 transfer efficiency from different surfaces to human skin and its survival on skin under varying temperatures.

Optimising the use of Oxford Nanopore Sequencing technology for detection of Wolbachia bacterial endosymbionts in Anopheles mosquitoes.

This study evaluates Oxford Nanopore Technology for detecting Wolbachia in Anopheles mosquitoes, finding it effective for pooled samples but not individual mosquitoes.

Mosquitoes reared in distinct insectaries within an institution in close spatial proximity possess significantly divergent microbiome

This study shows that mosquitoes reared in different insectaries have divergent microbiomes, influenced by environmental conditions and food sources.

Contrasting patterns of Asaia sp association with Plasmodium falciparum between field collected Anopheles gambiae and Anopheles coluzzii from Cameroon

This study quantifies Asaia sp. infection in Anopheles mosquitoes from Cameroon and finds no correlation between Asaia and Plasmodium falciparum infection.

Thermal variation influences the transcriptome of the major malaria vector Anopheles stephensi.

This study examines how temperature affects the transcriptome of Anopheles stephensi mosquitoes, identifying gene expression patterns and their implications for mosquito physiology and malaria transmission.

Recovery of Metagenomic Data from the Aedes aegypti Microbiome using a Reproducible Snakemake Pipeline: MINUUR

The paper develops a Snakemake pipeline called MINUUR to recover metagenomic data from Aedes aegypti genomic datasets, identifying bacterial MAGs and confirming known microbiome symbionts.

Genetic and microbial diversity of the invasive mosquito vector species Culex tritaeniorhynchus across its extensive inter-continental geographic range

This study examines genetic and microbial diversity of Culex tritaeniorhynchus across multiple continents, finding high genetic and microbial variation and evidence of Wolbachia and insect-specific viruses.

Integration of whole genome sequencing and transcriptomics reveals a complex picture of insecticide resistance in the major malaria vector Anopheles coluzzii

This study integrates whole genome sequencing and transcriptomics to identify multiple factors contributing to insecticide resistance in Anopheles coluzzii.

An integrated mosquito small RNA genomics resource reveals dynamic evolution and host responses to viruses and transposons.

The paper develops an integrated resource to analyze small RNA profiles in mosquitoes, revealing evolutionary dynamics and host responses to viruses and transposons.

Cas9-mediated gene-editing in the malaria mosquito Anopheles stephensi by ReMOT Control

The paper adapts ReMOT Control to edit genes in Anopheles stephensi mosquitoes, achieving editing efficiency comparable to embryo injections.

Multiplex logic processing isothermal diagnostic assays for an evolving virus

This paper develops a multiplex isothermal diagnostic assay for detecting variable viral targets, demonstrating its effectiveness against divergent Zika virus lineages.

Direct nucleic acid analysis of mosquitoes for high fidelity species identification and detection of Wolbachia using a cellphone

This paper develops a LAMP-OSD assay with cellphone imaging for rapid, accurate detection of mosquito species and Wolbachia infection in field samples.