Alain Kohl

Argonaute loss is associated with reduced endogenous leishbuvirid RNA abundance and disruption of viral small RNA accumulation in Leishmania martiniquensis

The paper investigates the role of Argonaute in controlling viral replication in Leishmania martiniquensis, finding that its loss reduces viral RNA abundance and disrupts small RNA accumulation.

sRNAMap: a lightweight, browser-based web application for small RNA mapping, analysis and visualisation

sRNAMap is a browser-based tool for analyzing and visualizing small RNA sequencing data, offering alignment, coverage, and feature quantification with high concordance to existing methods.

Emergence Risks from Within? Metagenomic Analysis of Mosquito Viromes from Two Zoos Reveals Mosquito-associated Orthobunyaviruses in the UK

The paper analyzes mosquito viromes from UK zoos using metagenomics, identifying two novel Orthobunyaviruses, including one potentially arbovirus.

Expanding Reverse Genetics of Positive-Strand RNA Viruses: Optimised Rescue Platforms and Construction of a Novel Fluorescent Reporter Nidovirus

The paper optimizes reverse genetics methods for positive-sense RNA viruses, showing improved recovery efficiency using specific promoters and ribozymes.

Leishmania guyanensis controls endogenous viral replication by a canonical RNA interference pathway

This study identifies Dicer-like 1 and Argonaute 1 as critical for controlling viral RNA levels in Leishmania guyanensis via a canonical RNA interference pathway.

Functional divergence of the NSs protein defines interferon antagonism and viral fitness across Bhanja virus lineages.

SFTSV NSs protein is a novel tick antiviral RNAi response suppressor

This study shows that SFTSV NSs protein is essential for viral replication in tick cells by suppressing the tick's RNAi response through depletion of virus-derived siRNAs.

Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cell cultures

This study identifies 283 RNA-binding proteins whose activity changes in tick cells infected with Uukuniemi virus, revealing new host-virus interactions and a method to silence gene expression in tick cultures.

Lack of Vector Competence in UK Culex pipiens molestus for Oropouche Virus

The paper tests if UK Culex pipiens molestus mosquitoes can transmit Oropouche virus, finding no evidence of infection in tested individuals.

Genetic diversity of Toscana virus glycoproteins affects the kinetics of virus entry and the infectivity of newly produced virions

This study investigates how genetic diversity in Toscana virus glycoproteins affects viral entry kinetics and infectivity, showing differences between two genetic lineages.

The PAZ domain of Aedes aegypti Dicer 2 is critical for accurate and high-fidelity size determination of virus-derived small interfering RNAs.

This study identifies the PAZ domain's role in ensuring accurate 21 nt vsiRNA production by Dcr2 in Aedes aegypti, critical for antiviral responses against Semliki Forest virus.

NSm is a critical determinant for bunyavirus transmission between vertebrate and arthropod hosts

This study identifies NSm as a critical protein for bunyavirus transmission between vertebrate and arthropod hosts, showing its necessity for midgut infection and spread.

The NS1 protein of contemporary West African Zika virus is efficient to increase cellular permissiveness to virus replication

This study compares NS1 proteins from contemporary and historical West African ZIKV strains, showing differences in secretion efficiency and virus replication impact.

The replication properties of a contemporary Zika virus from West Africa depends on NS1/NS4B proteins

This study compares replication properties of a West African ZIKV strain with an African strain, identifying NS1/NS4B proteins as key factors in replication and stress granule suppression.

periscope: Sub-Genomic RNA Identification in SARS-CoV-2 Genomic Sequencing Data

Periscope identifies and quantifies sub-genomic RNAs in SARS-CoV-2 genomic data, detecting canonical and non-canonical sgRNAs with high fidelity.

Zika virus-like particles bearing covalent dimer of envelope protein protect mice from lethal challenge

This paper develops ZIKV vaccines using virus-like particles with covalently dimerized envelope protein, showing enhanced neutralization and protection against lethal ZIKV challenge in mice.

Diverse laboratory colonies of Aedes aegypti harbor the same adult midgut bacterial microbiome

The paper compares the midgut bacterial microbiome of geographically diverse Aedes aegypti colonies reared in the same insectary, finding they are similar, suggesting microbiota is environmentally determined.

Advancing insect vector biology research: a community survey for future directions, research applications and infrastructure requirements

The paper summarizes survey findings on priorities and infrastructure needs for vector biology research, focusing on European countries.