Tyson A. Clark

Ultra high-throughput whole-genome methylation sequencing reveals trajectories in precancerous polyps to early colorectal adenocarcinoma

The paper uses ultra high-throughput methylation sequencing to study DNA methylation changes during colorectal cancer progression from normal tissue to adenocarcinoma.

Cost-efficient whole genome-sequencing using novel mostly natural sequencing-by-synthesis chemistry and open fluidics platform

Open flow cell on a circular wafer with mostly natural nucleotides and no reversible terminators: about 300 bp reads at $1/Gb, 99.6% SNP accuracy on reference human genomes.

High accuracy DNA sequencing on a small, scalable platform via electrical detection of single base incorporations

This paper introduces an electronic detection-based DNA sequencing platform that achieves high accuracy and scalability for microbial and human genome sequencing.

Targeted Long-Read RNA Sequencing Demonstrates Transcriptional Diversity Driven by Splice-Site Variation in MYBPC3

The paper uses targeted long-read RNA sequencing to identify transcriptional diversity caused by a splice-site variant in MYBPC3 associated with hypertrophic cardiomyopathy.

SMRT-Cappable-seq reveals complex operon variants in bacteria

SMRT-Cappable-seq reveals extended operons and read-through termination sites in E. coli, showing most bacterial genes are part of multiple operon variants.

Universal Alternative Splicing Of Noncoding Exons

The paper identifies that nearly all noncoding exons undergo alternative splicing, generating a vast repertoire of regulatory RNAs and highlighting conserved splicing patterns across species.

Amplification-free, CRISPR-Cas9 Targeted Enrichment and SMRT Sequencing of Repeat-Expansion Disease Causative Genomic Regions

Combining amplification-free CRISPR-Cas9 targeted enrichment with Single Molecule, Real-Time (SMRT) long-read sequencing captures and sequences human repeat-expansion disease loci across HTT, FMR1, C9orf72, and ATXN10 without PCR bias.