Author

Steven Huysecom

KU LeuvenORCID 0000-0002-7939-3913Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Research Focus:cell signaling & trafficking· 3 Research Focus:drug response / drug discovery· 2 Approach:experimental assay· 2 Research Focus:cell type annotation· 1 Approach:deep learning· 1 Research Focus:development & morphogenesis· 1 Assay:fluorescence / live-cell microscopy· 1 Organism:human· 1

Frequent Co-Authors

Susana RochaiD· 3 shared Beatrice FortuniiD· 2 shared Boris LouisiD· 2 shared Indra Van ZundertiD· 2 shared Sandra KrzyzowskaiD· 2 shared Ana Montero‐CalleiD· 1 shared G. Solís-FernandeziD· 1 shared Guillermo Solís‐FernándeziD· 1 shared

Activity by Year

2025-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 32 mutual co-author links

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3 shared papersCell Biology

Susana RochaORCID

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3 papers

Recent·Most cited

When lysosomes persist: resolving the proton-sponge paradox in nanoparticle-based intracellular delivery

Indra Van Zundert, Steven Huysecom, Thibo Iven, Sandra Krzyzowska, Vince Goyvaerts, Volker Leen, Johan Hofkens, Hiroshi Uji‐i, Beatrice Fortuni, Susana Rocha

This study investigates how proton-sponge polymers like PEI enable cargo release without full nanoparticle escape, resolving a long-standing debate in nanomedicine.

bioRxiv · May 7, 2026 · PreprintCell Biology

Quantitative Analysis of Cytoplasmic Viscosity in Colorectal Cancer Cells by Differential Dynamic Microscopy of Genetically Encoded Nanoparticles

Steven Huysecom, Kiki Schouwstra, Samet Aytekin, Natalie Neyrinck, Yana Heyvaert, Boris Louis, Susana Rocha, Guillermo Solís‐Fernández

This paper introduces GEM-DDM, a method using genetically encoded nanoparticles and differential dynamic microscopy to measure cytoplasmic viscosity in living cells, showing increased viscosity in aggressive colorectal cancer cells.

Chemical & Biomedical Imaging · Apr 10, 2026Peer-reviewedBiophysics

Beyond One-Size-Fits-All: Tumor Biology Influences Nanoparticle Behaviour in Cancer Models

M. Bravo, G. Solís-Fernandez, Sandra Krzyzowska, Steven Huysecom, Ana Montero‐Calle, Indra Van Zundert, Boris Louis, Rodrigo Barderas, Beatrice Fortuni, Susana Rocha

This study shows that nanoparticles behave differently in various tumor models, emphasizing the need to tailor their design based on tumor biology.

bioRxiv · Mar 26, 2025 · PreprintCell Biology