Johan Hofkens

Synthetic Fibrous Hydrogels as Minimal Systems to Modulate Cell Migration Modes in 3D

This paper introduces tunable fibrous hydrogels to study how specific ligands direct different cell migration modes in 3D environments.

Unlocking subcellular imaging of a cnidarian photosymbiont Breviolum minutum , through expansion microscopy

This paper develops an expansion microscopy workflow to visualize subcellular features of Breviolum minutum, a cnidarian photosymbiont, overcoming challenges posed by autofluorescence and small size.

When lysosomes persist: resolving the proton-sponge paradox in nanoparticle-based intracellular delivery

This study investigates how proton-sponge polymers like PEI enable cargo release without full nanoparticle escape, resolving a long-standing debate in nanomedicine.

ABEL-FRET bridges the timescale gap in single-molecule measurements of the structural dynamics in the A2A adenosine receptor

This study uses ABEL-FRET to measure structural dynamics of A2A adenosine receptors across timescales, showing agonists shift receptor states and accelerate conformational exchange.

Nanoparticle Accumulation and Penetration in 3D Tumor Models: the Effect of Size, Shape, and Surface Charge

This study evaluates how nanoparticle size, shape, and surface charge affect their accumulation and penetration in 2D and 3D tumor models, finding that larger, negatively charged, and spherical NPs perform best.

DynaMAP: Dynamic Microbiome Abundance Profiling through high density optical mapping

DynaMAP provides rapid, strain-level microbiome profiling using optical mapping, achieving results comparable to shotgun sequencing in one day.

Identifying microbial species by single-molecule DNA optical mapping and resampling statistics

This study improves the sensitivity and specificity of microbial species identification using single-molecule DNA optical mapping and resampling statistics.

Nucleotide-mediated allosteric regulation of bifunctional Rel enzymes

This study reveals a guanosine-switch mechanism that regulates the opposing activities of bifunctional Rel enzymes through allosteric changes.