Kin Chung Lam

The paper introduces PERSIST-On, a CRISPR-based method for stable and tunable gene activation by recruiting endogenous transcription factors to target promoters.

MOSAIC enables high-throughput in situ saturation mutagenesis and CRISPR prime editing guide RNA optimization in human cells, identifying optimized pegRNAs and drug resistance variants.

PrimeDesign is a tool for designing prime editing guide RNAs, enabling single and combination edits, and it supports the creation of a database for over 68,500 pathogenic variants.

This study shows the NSL complex maintains nucleosome organization at promoters, ensuring transcription fidelity and suppressing noise by preventing nucleosome invasion into NDRs.

The paper identifies DNA motifs at TAD boundaries in flies and shows they encode 3D genome architecture, with an online database for exploration.