Nature CommunicationsBioinformaticsPeer-reviewed journal article
PrimeDesign software for rapid and simplified design of prime editing guide RNAs
Jonathan Y. HsuiD, Julian GrünewaldiD, Regan N. SzalayiD, Justine ShihiD, Andrew V. AnzaloneiD, Kin Chung LamiD, Max W. SheniD, Karl PetriiD, David R. LiuiD, J. Keith JoungiD, Luca PinelloiD
Abstract
Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web application and command-line tool for the design of PE experiments. PrimeDesign can be used for single and combination editing applications, as well as genome-wide and saturation mutagenesis screens. Using PrimeDesign, we also constructed PrimeVar, the first comprehensive and searchable database for prime editing guide RNA (pegRNA) and nicking sgRNA (ngRNA) combinations to install or correct >68,500 pathogenic human genetic variants from the ClinVar database.
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