Liang Shan

This study uses reverse genetics in humanized mice to show that DPP9 deficiency causes CARD8-mediated pyroptosis in human hematopoietic stem and progenitor cells, leading to pancytopenia.

This paper establishes a method to generate humanized mice with targeted gene knockouts in the human immune system using Cas9-edited CD34+ cells.

The paper identifies CD34 as an intrinsic innate immune factor that restricts retrovirus replication in stem cells by inactivating progeny virions.