Jingyi Fei

Super-resolved spatial organization of the nucleolar transcriptome

The paper uses super-resolution imaging and in situ sequencing to map RNA distribution within nucleolar subdomains, revealing distinct RNA populations and processing dynamics.

RNA localization to nuclear speckles follows splicing logic

This study identifies sequence features that determine RNA localization to nuclear speckles, showing similarities with splicing codes and linking speckle localization to spliceosome assembly and splicing efficiency.

Transcriptome-wide mRNP condensation precedes stress granuleformation and excludes new mRNAs

The paper shows that mRNAs condense broadly in yeast under stress, independent of length, and new transcripts escape this condensation for selective translation.

Effects of individual base-pairs on in vivo target search and destruction kinetics of small RNA

This study investigates how single base-pair changes affect the in vivo kinetics of sRNA-target interactions, showing that Hfq facilitates annealing and buffers base-pair disruptions.

Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9

The paper uses single-molecule FRET to study how mismatches affect Cas9-RNA DNA binding and rejection kinetics, revealing that proximal mismatches lead to rapid rejection while distal mismatches allow stable complex formation.