Author

Ruud F. G. Toonen

Vrije Universiteit AmsterdamORCID 0000-0002-9900-4233Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 8 Organism:mouse· 6 Research Focus:cell type annotation· 3 Research Focus:cell-cell communication· 2 Assay:electronic health records· 2 Organism:human· 2 Assay:bulk RNA-seq· 1 Research Focus:differential expression· 1

Frequent Co-Authors

Matthijs VerhageiD· 8 shared August Benjamin SmitiD· 3 shared Jan R.T. van WeeringiD· 3 shared Fiona H MurphyiD· 2 shared Rein I. Hoogstraaten· 2 shared Adlin AbramianiD· 1 shared Alexander J. A. GroffeniD· 1 shared Annemiek A. van BerkeliD· 1 shared

Activity by Year

2020-2025 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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Curved chords = mutual co-authorship

8 shared papersNeuroscience

Matthijs VerhageORCID

Mutual co-authors in cluster

Author profile →

8 papers

Recent·Most cited

One-step induction of human GABAergic neurons promotes presynaptic development & synapse maturation

Torben W. van Voorst, Maaike A. van Boven, Kevin I. Marinus, J Colon, Jule Schretzmeir, Carolin Haag, Ruud F. G. Toonen, Frank Koopmans, Michael Emmerson Ward, August Benjamin Smit, Ronald E. van Kesteren, Matthijs Verhage, Lennart Niels Cornelisse

This study develops a single-step transposon-based method to generate pure GABAergic neurons from iPSCs, showing they mature synaptically and form functional networks.

bioRxiv · Jun 30, 2025 · PreprintNeuroscience

Rab10 regulates neuropeptide release by maintaining Ca2+ homeostasis and protein synthesis

Jian Dong, Miao Chen, Jan R.T. van Weering, Natalia Domínguez, Ka Wan Li, August Benjamin Smit, Ruud F. G. Toonen, Matthijs Verhage

This study shows Rab10 maintains Ca2+ homeostasis and protein synthesis to regulate neuropeptide release via DCVs in mouse hippocampal neurons.

eLife · Apr 2, 2025Peer-reviewedNeuroscience

ER Ca2+-levels control neuromodulator secretion by regulating L-type Ca2+-channel activity via its STIM1-interacting domain

Rein I. Hoogstraaten, Ruud F. G. Toonen, Matthijs Verhage

The paper shows that ER Ca2+ depletion in mouse neurons reduces DCV exocytosis by inhibiting L-type Ca2+ channels via a STIM1-dependent feedback loop, while not affecting SV exocytosis.

bioRxiv · Nov 12, 2024 · PreprintNeuroscience

Rabphilin-3A negatively regulates neuropeptide release, through its SNAP25 interaction

Adlin Abramian, Rein I. Hoogstraaten, Fiona H Murphy, Kathryn F McDaniel, Ruud F. G. Toonen, Matthijs Verhage

This study identifies rabphilin-3A as a negative regulator of DCV exocytosis through its interaction with SNAP25, showing increased DCV release in its absence.

eLife · Oct 16, 2024Peer-reviewedNeuroscience

KIF5A regulates axonal repair and time-dependent axonal transport of SFPQ granules and mitochondria in human motor neurons

Irune Guerra San Juan, Jessie Brunner, Kevin C. Eggan, Ruud F. G. Toonen, Matthijs Verhage

This study investigates the role of KIF5A in axonal transport and repair in human motor neurons, finding that its loss impairs mitochondrial and SFPQ granule transport and axonal regeneration.

bioRxiv · Sep 6, 2024 · PreprintNeuroscience

SNARE protein tomosyn regulates dense core vesicle composition but not exocytosis in mammalian neurons

Aygul Subkhangulova, Miguel A. Gonzalez‐Lozano, Alexander J. A. Groffen, Jan R.T. van Weering, August Benjamin Smit, Ruud F. G. Toonen, Matthijs Verhage

This study shows that tomosyn regulates DCV cargo levels but not exocytosis in neurons, suggesting a role in cargo production rather than release.

eLife · Aug 29, 2023Peer-reviewedNeuroscience

Differential axonal trafficking of Neuropeptide Y-, LAMP1- and RAB7-tagged organelles in vivo

Joris P Nassal, Fiona H Murphy, Ruud F. G. Toonen, Matthijs Verhage

The paper investigates the trafficking of three organelle markers in thalamocortical axons in vivo, finding differences in speed and behavior compared to in vitro studies.

eLife · Nov 24, 2022Peer-reviewedNeuroscience

Retrograde transport defects in Munc18-1 null neurons explain abnormal Golgi morphology

Annemiek A. van Berkel, Tatiana C. Santos, Hesho Shaweis, Jan R.T. van Weering, Ruud F. G. Toonen, Matthijs Verhage

This study identifies retrograde transport defects in MUNC18-1 null neurons as the cause of abnormal Golgi morphology and neurodegeneration.

bioRxiv · May 12, 2020 · PreprintNeuroscience