Guoling Li

The paper creates a spatiotemporal transcriptome atlas of human embryos after gastrulation, revealing organ-specific regulons and regulatory networks during development.

This paper develops two deaminase-free base editors, gTBE and gCBE, for direct T and C editing using engineered UNG variants, achieving high editing efficiencies in human cells.

This paper develops a system to package mRNAs into AAVs, enabling efficient whole-brain mRNA delivery in mice.

This paper engineers a TnpB-ωRNA system for efficient gene editing in mice, showing improved activity over Cas12f1 and successful disease prevention in a tyrosinaemia model.

The paper designs high-fidelity Cas13 variants with minimal collateral RNA degradation effects, validated in mammalian cells and transgenic mice.

This paper describes the generation of multi-transgenic pigs expressing digestive enzymes to improve feed efficiency and reduce environmental impact.

This study establishes Etv5 gene knockout mice as a recipient model for spermatogonial stem cell transplantation, showing partial success in germ cell restoration but low efficiency in full spermatogenesis.