Yingsi Zhou

Development of deaminase-free T-to-S base editor and C-to-G base editor by engineered human uracil DNA glycosylase

This paper develops two deaminase-free base editors, gTBE and gCBE, for direct T and C editing using engineered UNG variants, achieving high editing efficiencies in human cells.

Multi-step engineered adeno-associated virus enables whole-brain mRNA delivery

This paper develops a system to package mRNAs into AAVs, enabling efficient whole-brain mRNA delivery in mice.

Engineering transposon-associated TnpB-1 ωRNA system for efficient gene editing and disease treatment in mouse

This paper engineers a TnpB-ωRNA system for efficient gene editing in mice, showing improved activity over Cas12f1 and successful disease prevention in a tyrosinaemia model.

Interspecies blastocyst complementation generates functional rat cell-derived forebrain tissues in mice

This study uses interspecies blastocyst complementation to generate functional rat-derived forebrain tissues in mice, revealing developmental and xenogeneic barriers.

High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effect

The paper designs high-fidelity Cas13 variants with minimal collateral RNA degradation effects, validated in mammalian cells and transgenic mice.

Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver

This study demonstrates the use of CasRx-mediated RNA knockdown to modulate metabolic genes in mouse liver, showing efficient and reversible reduction of PCSK9 and cholesterol levels.

CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration