The paper develops Biolearn, an open-source framework for curating and evaluating aging biomarkers across datasets, finding that harmonization and standardization improve their performance and generalizability.

Nature Aging · Nov 4, 2025Peer-reviewedBioinformatics

Multi-omics analysis reveals a crucial role for Retinoic Acid in promoting epigenetic and transcriptional competence of an in vitro model of human Pharyngeal Endoderm.

Andrea Cipriano, Alessio Colantoni, Danielle Gomes, Mahdi Moqri, Alexander Parker, Matthew Caldwell, Francesca Briganti, Jonathan Fiorentino, Maria Grazia Roncarolo, Antonio Baldini, Katja Gabriele Weinacht, Gian Gaetano Tartaglia, Vittorio Sebastiano

The paper develops an in vitro protocol to generate human Pharyngeal Endoderm cells and uses multi-omics analysis to reveal Retinoic Acid's role in their epigenetic and transcriptional development.

bioRxiv · Jun 26, 2022 · PreprintDevelopmental Biology

PRC2 clock: a universal biomarker of aging and rejuvenation

Mahdi Moqri, Andrea Cipriano, Daniel C. Nachun, Tara Murty, Guilherme De Sena Brandine, Sajede Rasouli, Andrei E. Tarkhov, Karolina A. Åberg, Edwin J. C. G. van den Oord, Wanding Zhou, Andrew D. Smith, Crystal Lee Mackall, Vadim N. Gladyshev, Steve Horvath, Michael P. Snyder, Vittorio Sebastiano

The paper identifies PRC2 low-methylated regions as a universal biomarker of aging and rejuvenation, showing they account for most age-related DNA methylation gain across tissues.

bioRxiv · Jun 3, 2022 · PreprintGenomics