Wanding Zhou

The paper develops and validates Sparse-Seq, a low-coverage sequencing method for accurate quantification of 5mC and 5hmC levels, showing it is more accessible and less variable than mass spectrometry.

The paper introduces scDEEP-mC, a single-cell DNA methylation profiling method, and shows it can identify cell types, X-inactivation states, and replication dynamics with high coverage.

The paper introduces MSA, a new epigenotyping platform for analyzing ternary-code DNA methylation, and applies it to uncover aging dynamics and trait associations involving 5hmC and 5mC.

This paper introduces a method for spatial co-profiling of DNA methylation and transcriptome in mammalian tissues, revealing spatial patterns and their interplay in development and brain function.

This study compares DNA methylation profiles of breast tumors, adjacent normal tissues, and healthy controls to identify epigenetic differences and their association with estrogen receptor status.

This study shows UHRF1 ubiquitin ligase activity and DNMT1 ubiquitin reading activity are essential for maintaining low-density CpG methylation, and their disruption leads to hypomethylation and PMD formation in cancer.

This study compares epigenetic aging rates between lab-reared and rewilded mice, finding faster aging in rewilded mice with implications for environmental influences on aging.

The paper develops a custom Infinium DNA methylation array for the human imprintome and validates its performance against WGBS and EPIC arrays.

mLiftOver converts DNA methylation data between Infinium platforms, enabling accurate integration and analysis across different array versions.

The paper develops low-input and single-cell methods for Infinium DNA methylation BeadChips, improving detection rates and data quality for small sample sizes.

This paper develops a high-throughput DNA methylation array and atlas for mice, revealing tissue-specific methylation patterns and their associations with aging, differentiation, and disease.

The paper identifies PRC2 low-methylated regions as a universal biomarker of aging and rejuvenation, showing they account for most age-related DNA methylation gain across tissues.

The paper develops a mammalian methylation array to profile methylation levels at conserved sequences across species, enabling cross-species comparisons and epigenetic biomarker development.

The paper investigates the role of EHMT2 in transcriptional regulation during mouse embryonic development, finding it controls transcriptional noise and developmental gene switches.

The paper identifies cell type-specific chromatin loops anchored at large DNA methylation nadirs, revealing their role in stem and progenitor cell function.

The paper investigates how 3D chromatin interactions regulate HOXA cluster genes in HSCs and leukemia by analyzing the role of the Geneless Canyon region.