Vadim N. Gladyshev

Transcriptomic rejuvenation during intestinal and liver regeneration in mice

This study shows that tissue regeneration in mice reduces transcriptomic age temporarily, with variations depending on tissue type and repair stage.

Systemic control and inter-organ communication of ovarian aging

This study shows ovarian aging can shift in both directions depending on the systemic environment through inter-organ communication and molecular changes.

Aged white adipose tissue is rejuvenated in a young host through thermogenic remodeling of mature adipocytes

This study shows that old white adipose tissue can rejuvenate in young hosts through thermogenic remodeling and pathway activation, reversing biological age markers.

A genome-scale ORF screen for resilience factors that protect the RPE and restore vision in aged mice

The paper identifies resilience factors in aged mice using a genome-scale ORF screen, finding NKX2-5 variants that restore vision and reduce frailty.

Transplanted hearts assimilate the recipient's biological age

DNA methylation clocks show heterochronic heart grafts shifting toward recipient age in syngeneic mice and 11 human recipients, while recipient mouse native heart, liver and blood show no consistent change.

Causally measuring aging and rejuvenation through transcriptomic damage

This paper introduces a computational framework to quantify transcriptomic damage across tissues and conditions, revealing age-associated increases and demonstrating that interventions can alter tDamAge.

Mammalian aging involves genome-wide splicing degeneration leading to functional decline

This study identifies splicing degeneration as a hallmark of aging, showing it increases with age and is reversible with calorie restriction or rapamycin.

Architectural fragility of gene regulatory networks underlies hematopoietic stem cell aging

The paper analyzes single-cell multiome data from human HSPCs to show aging-related rewiring of gene regulatory networks, leading to stress hyperactivation, myeloid bias, and identity loss.

An Open Competition for Biomarkers of Aging

The paper presents results from an open competition to develop aging biomarkers using DNA methylation data, showing improved predictive models for age, mortality, and multi-morbidity.

Comparative analysis of naked mole-rat thermogenesis and its potential to maintain euthermia in response to cold

This study investigates the thermogenic potential of naked mole-rat brown adipose tissue and its ability to maintain body temperature in cold, finding that NMRs can initiate non-shivering thermogenesis but cannot sustain it due to heat loss and substrate limitations.

Quantification of domain-specific intrinsic capacity using mortality data

This study uses UK Biobank data to develop an 18-year mortality risk estimator for domain-specific intrinsic capacity, identifying immune system activation across domains.

Thermodynamic scaling of canine aging and reversible clock deceleration by a reverse transcriptase inhibitor

The paper tracks aging in sled dogs using a variational autoregressive model to show that lamivudine temporarily decelerates DNA methylation clocks but not long-term biological age.

Balance between DNA repair, LINE1 suppression and lifespan in mice with SIRT6 Serine 10 mutations

This study shows that SIRT6 Serine 10 mutations in mice affect DNA repair, LINE1 expression, and lifespan, with reduced LINE1 control having a stronger impact on lifespan than enhanced DNA repair.

In vivo chemical reprogramming is associated with a toxic accumulation of lipid droplets hindering rejuvenation

This study shows that partial chemical reprogramming in aged mice increases lipid droplet formation, which may cause toxicity and hinder tissue rejuvenation.

Integrative epigenetics and transcriptomics identify aging genes in human blood

This study identifies aging genes in human blood by integrating epigenetic and transcriptomic data, finding genes enriched for immune functions that better replicate across populations and link to aging outcomes.

Conserved Master Regulators Orchestrate Cellular Reprogramming-Induced Rejuvenation

The paper identifies conserved master regulators that coordinate reprogramming-induced rejuvenation across different cell types and shows that perturbing these regulators can modulate transcriptomic age.

Multi-tissue spatial transcriptomics reveals biological age hotspots in mouse and human aging

The paper introduces stAge, a framework to quantify localized transcriptomic age from spatial transcriptomics data, revealing spatial gradients and age acceleration in mouse and human aging.

A Unified Framework for Systematic Curation and Evaluation of Aging Biomarkers

The paper develops Biolearn, an open-source framework for curating and evaluating aging biomarkers across datasets, finding that harmonization and standardization improve their performance and generalizability.

IPSC-based modeling of resiliency in centenarians reveals longevity-specific signatures

The paper uses iPSC-derived neurons from centenarians to identify resilience signatures associated with longevity, showing enhanced stress response and specific transcriptional programs.

Reprogramming Factors Activate a Non-Canonical Oxidative Resilience Pathway That Can Rejuvenate RPEs and Restore Vision

OSK factors rejuvenate aged RPE cells by activating a Tet2-independent oxidative resilience pathway through GSTA4, restoring vision and reversing age-related degeneration.

The 18S rRNA Methyltransferase DIMT-1 Regulates Lifespan in the Germline Later in Life

This study identifies DIMT-1 as a regulator of C. elegans lifespan and stress resistance through its role in rRNA methylation and germline function.

DNA Methylation Ageing Atlas Across 17 Human Tissues

The paper creates a DNA methylation aging atlas across 17 human tissues, identifying conserved and tissue-specific aging signatures and potential therapeutic targets.

Profiling Epigenetic Aging at Cell-Type Resolution Through Long-Read Sequencing

This paper introduces LongReadAge, a probabilistic model for predicting epigenetic age at the cell-type level using long-read sequencing data.

Molecular signatures of longevity identify compounds that extendmouse lifespan and healthspan

The paper identifies compounds that extend mouse lifespan and healthspan using a biomarker-driven platform integrating gene expression, chemical screening, and lifespan studies.

Non-DNA-damaging DNA-PK activation improving hearing and prolonging life due to NAD+ and SIRT upregulation

This paper shows that MA-5 improves hearing loss and extends lifespan in mice by increasing NAD+ and SIRT1 through DNA-PK activation and mitochondrial function.

Engineering ATP Import in Yeast Uncovers a Synthetic Route to Extend Cellular Lifespan

This paper shows that manipulating intracellular ATP levels in yeast through synthetic ATP import extends cellular lifespan and reveals distinct metabolic pathways involved in aging.

Regeneration leads to global tissue rejuvenation in aging sexual planarians

Regeneration in aging planarians reverses age-related physiological and molecular changes, restoring youthful gene expression and tissue composition.

A torpor-like state (TLS) in mice slows blood epigenetic aging and prolongs healthspan

This study shows that inducing a torpor-like state in mice slows blood epigenetic aging and improves healthspan by reducing core body temperature.

Methionine sulfoxide reductase B3 antioxidant activity is indispensable for mouse inner ear cuticular plate structure and hair bundle integrity

This study shows that MSRB3 is essential for maintaining inner ear structure and hair bundle integrity in mice, with its antioxidant activity protecting actin redox dynamics.

A single cell atlas of the mouse seminal vesicle

This study creates a single-cell RNA-seq atlas of the mouse seminal vesicle, identifying secretory and immune cell populations and their transcriptomes.