Karole N. D’Orazio

The paper investigates mRNA quality control pathways in yeast, showing that Syh1 is central to no-go decay (NGD) while Not5 has a minor role, and ribosome profiling reveals distinct ribosomal states for NGD and COMD.

The paper identifies factors linking ribosome recycling and nonsense mediated decay in yeast using genetic screens and reporter constructs.

The paper identifies GIGYF2 and 4EHP as translation inhibitors that suppress re-initiation of defective mRNAs, aiding ribosome-associated quality control and preventing toxic protein accumulation.

The paper identifies Cue2 as the endonuclease responsible for cleaving mRNAs at stalled ribosomes during No Go Decay and shows it acts as a secondary decay pathway.