Dario Boffelli

This study identifies synthesis errors in ssODNs used for HDR-mediated gene editing and shows they propagate into the genome, potentially causing disease alleles.

This study shows that IL-25 induces memory ILC2s in the small intestine that provide long-term protection against barrier pathogens.

The paper shows partially differentiated CFTR-mutant enteroids secrete fluid in response to forskolin and linaclotide, with reduced efficacy compared to healthy enteroids.

The paper shows Pdgfra+ fibroblasts reprogram during WNT-driven oncogenesis to promote a regenerative state in the epithelium, facilitating tumor formation.

This study identifies an active mechanical mechanism involving PDGFRA+ mesenchymal cells that pattern and fold intestinal villi through myosin II-dependent forces and matrix metalloproteinase-mediated tissue fluidization.

The paper identifies five discrete metabolic domains in the mouse and human small intestine using transcriptional signatures and machine learning, showing how diet influences domain properties.

This study evaluates the Full Genome Analysis method for diagnosing rare monogenic disorders, finding a 40% diagnostic yield in clinical cases.

This study uses CRISPR-Cas9 to test a putative fetal globin repressor region, finding it does not autonomously silence gamma-globin, suggesting it is not a therapeutic target for beta-hemoglobinopathies.

Cas9-mediated correction of the sickle mutation in human hematopoietic stem cells achieves curative levels of editing.