Shreeram Akilesh

This study uses human precision cut kidney slices to analyze gene expression responses to culture conditions and IFNγ stimulation, showing reproducible APOL1 expression changes.

This study shows that CD19 CAR-engineered regulatory T cells reduce B cell numbers, inflammation, and autoantibodies in lupus models, promoting long-term immune tolerance.

This study evaluates the rigor and reproducibility of digital spatial profiling in clinically sourced human tissues, finding it to be highly reliable.

The paper identifies medulla-specific gene expression patterns in adult human kidney tissues using multi-omic data and corrects misassignments in the GTEx database.

The paper defines the chromatin landscape of healthy and injured kidney cells using multi-omics data, identifying regulatory elements and transcription factor networks involved in injury response.

The paper introduces pSABER, a method for amplifying in situ hybridization signals in cellular and histopathological samples, achieving 25-fold amplification over conventional methods.

PaintSHOP provides an interactive platform for designing oligonucleotide FISH experiments, integrating machine learning to predict probe specificity and generate standardized probe sets.