Thomas Vierbuchen

The paper identifies that X chromosome dosage influences aneuploidy development in mouse embryonic stem cells, with XO and XY lines showing higher aneuploidy rates than XX lines.

This study shows NIPBL-mediated 3D genome folding is essential for enhancer-promoter communication and maintaining lineage fidelity during embryonic development.

ASPEN improves detection of allelic dynamics in single-cell RNA-seq by modeling mean and variance, revealing patterns of allelic imbalance and variance in mouse cells.

The paper develops a mouse cortical organoid platform to study cis-regulatory evolution and identifies genes with dynamic allelic imbalances during neurogenesis.

This paper develops a protocol to efficiently generate tissue-resident macrophages from mouse pluripotent stem cells, enabling in vitro and in vivo modeling of their development.

The paper identifies genetic variants that influence DNA methylation stability and imprint deregulation in naive pluripotent stem cells across different mouse strains.

This paper establishes a pluripotent stem cell platform using genetically diverse mouse lines to study the impact of natural genetic variation on development and gene regulation.

The paper uses a CRISPRi screen in hESC differentiation to identify enhancers with nonlinear regulatory activity, revealing a CTCF loop-constrained model for improved enhancer prediction.

The paper identifies sequence variants affecting enhancer function by analyzing TF binding and activity in hybrid mice fibroblasts.

The paper presents optimized protocols for generating definitive endoderm and forebrain-patterned neural organoids from mouse epiblast stem cells.

The paper identifies a bipartite imprinting control region at the Dlk1-Dio3 locus that maintains imprint stability through allele-specific regulation of DNA methylation and chromatin structure.