snPATHO-seq: unlocking the pathology archives

Taopeng Wang; Kate Harvey; Javier Escudero Morlanes; Beata Kiedik; Ghamdan Al‐Eryani; Alissa Greenwald; Nikolaos Kalavros; Felipe Segato Dezem; Yuling Ma; Yered H. Pita-Juárez; Kellie Wise; Cyril Dégletagne; Anna Elz; Azi Hadadianpour; John B. Johanneson; Fiona J. Pakiam; Heeju Ryu; Evan William Newell; Laurie Tonon; Andrew S. Kohlway; Tingsheng Drennon; Jawad Abousoud; Ryan T. Stott; Paul E. Lund; Jens Durruthy-Durruthy; Andrés F. Vallejo; Dominik C. Kaczorowski; Joanna Warren; Lisa M. Butler; Sandra A O'Toole

doi:10.1101/2023.12.07.570700

Molecular BiologybioRxivDec 7, 2023

Preprint

snPATHO-seq: unlocking the pathology archives

Taopeng Wang iD, Kate Harvey iD, Javier Escudero Morlanes iD, Beata Kiedik iD, Ghamdan Al‐Eryani iD, Alissa Greenwald iD, Nikolaos Kalavros iD, Felipe Segato Dezem iD, Yuling Ma iD, Yered H. Pita-Juárez iD, Kellie Wise iD, Cyril Dégletagne iD, Anna Elz iD, Azi Hadadianpour, John B. Johanneson iD, Fiona J. Pakiam iD, Heeju Ryu iD, Evan William Newell iD, Laurie Tonon iD, Andrew S. Kohlway, Tingsheng Drennon, Jawad Abousoud, Ryan T. Stott, Paul E. Lund iD, Jens Durruthy-Durruthy, , Luciano Gastón Martelotto iD

Abstract

Formalin-fixed paraffin-embedded (FFPE) samples are valuable but underutilized in single-cell omics research due to their low DNA and RNA quality. In this study, leveraging recent single-cell genomic technology advances, we introduce a versatile method to derive high-quality single-nucleus transcriptomic data from FFPE samples.

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