Nature CommunicationsSynthetic BiologyPeer-reviewed journal article
Activation of stably silenced genes by recruitment of a synthetic de-methylating module
Wing Fuk ChaniD, Hannah D. CoughlaniD, Yunshun CheniD, Christine R. KeenaniD, Gordon K. SmythiD, Andrew Charles PerkinsiD, Timothy M. JohansoniD, Rhys S. AllaniD
Abstract
Stably silenced genes that display a high level of CpG dinucleotide methylation are refractory to the current generation of dCas9-based activation systems. To counter this, we created an improved activation system by coupling the catalytic domain of DNA demethylating enzyme TET1 with transcriptional activators (TETact). TETact induces transcription of heavily suppressed non-coding RNA and surface protein, and the reactivation of embryonic haemoglobin genes in non-erythroid cells.
- assay: DNA methylation
- research focus: gene regulatory network inference
- research focus: variant effect prediction
- approach: experimental assay
- Citations
- 26
- FWCI
- 1.47
- Field percentile
- top 18%
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Casilio-ME: Enhanced CRISPR-based DNA demethylation by RNA-guided coupling methylcytosine oxidation and DNA repair pathways
Aziz Taghbalout, Menghan Du, … Albert W. Cheng·Nature CommunicationsPeer-reviewed·Sep 20, 2019
- Components from the human c-myb transcriptional regulation system reactivate epigenetically repressed transgenes
Cassandra M. Barrett, Reilly McCracken, … Karmella Ann Haynes·International Journal of Molecular SciencesPeer-reviewed·Jan 14, 2020
- Reversing transgene silencing via targeted chromatin editing
Elia Salibi, Sebastian Palacios, … Domitilla Del Vecchio·bioRxiv·Oct 28, 2025
- Unraveling the functional role of DNA methylation using targeted DNA demethylation by steric blockage of DNA methyltransferase with CRISPR/dCas9
Daniel M. Sapozhnikov, Moshe Szyf A·Nature CommunicationsPeer-reviewed·Sep 29, 2021
- Targeted intragenic demethylation initiates chromatin rewiring for gene activation
Yanjing V. Liu, Mahmoud Adel Bassal, … Daniel Geoffrey Tenen·bioRxiv·Jul 16, 2020
- Natural depletion of H1 in sex cells causes DNA demethylation, heterochromatin decondensation and transposon activation
S. He, M. Vickers, … X. Feng·bioRxiv·Oct 26, 2018