A Cre-amplifier to generate and detect genetic mosaics in vivo

Francesco Trovato; Riccardo Parra; Enrico Pracucci; Silvia Landi; Olga Cozzolino; Gabriele Nardi; Federica Cruciani; Laura Mosti; Andrzej Cwetsch; Laura Cancedda; Laura Gritti; Carlo Sala; Chiara Verpelli; Andrea Maset; Claudia Lodovichi; Gian Michele Ratto

doi:10.1101/715490

NeurosciencebioRxivJul 26, 2019

Preprint

A Cre-amplifier to generate and detect genetic mosaics in vivo

Francesco Trovato iD, Riccardo Parra iD, Enrico Pracucci iD, Silvia Landi iD, Olga Cozzolino iD, Gabriele Nardi iD, Federica Cruciani iD, Laura Mosti iD, Andrzej Cwetsch, Laura Cancedda iD, Laura Gritti iD, Carlo Sala iD, Chiara Verpelli iD, Andrea Maset iD, Claudia Lodovichi iD, Gian Michele Ratto iD

Abstract

Cre-Lox manipulation is the gold standard for cell-specific expression or knockout of selected genes. However, it is not unusual to deal with conditions of low Cre expression or transient activation, which can often go undetected by conventional Cre-reporters. We designed Beatrix, a general-purpose tool specifically devised to amplify weak Cre recombinase activity, and we used it to develop a powerful approach for the in vivo generation and detection of sparse mosaics of mutant and wild type cells.

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