Gabriele Nardi

iClima: a ratiometric genetically encoded sensor for optical imaging of fast GABAergic chloride currents in vitro and in vivo

The paper introduces iClima, a ratiometric genetically encoded sensor for imaging GABAergic chloride currents, showing it can detect Cl- transients in vitro and in vivo with improved photostability and pH independence.

Sleep deprivation impairs information processing via dysregulation of chloride homeostasis in the prefrontal cortex

This study shows sleep deprivation impairs information processing in the prefrontal cortex by disrupting chloride homeostasis through KCC2 reduction and BDNF-TrkB signaling, which can be mitigated by bumetanide or AP synthesis blockade.

Circadian rhythm in cortical chloride homeostasis underpins variation in network excitability

The paper shows that intracellular chloride levels in cortical pyramidal cells vary diurnally, affecting neuronal network stability and seizure latency.

A Cre-amplifier to generate and detect genetic mosaics in vivo

The paper introduces Beatrix, a tool to amplify weak Cre activity for generating and detecting genetic mosaics in vivo.