Alexandros Poulopoulos

Developmental transformations of Purkinje cells tracked by DNA electrokinetic mobility

The paper uses stereo-tracking to study Purkinje cell development and identifies a new subcellular structure called axon bubbles.

BEAM: a combinatorial recombinase toolbox for binary gene expression and mosaic analysis

BEAM generates two genetically distinct cell populations for mosaic analysis by using sparse recombinase activation to label cells with green or red fluorescent protein.

Symmetry in levels of axon-axon homophilic adhesion establishes topography in the corpus callosum and development of connectivity between brain hemispheres

The paper identifies topographic principles in the corpus callosum that guide axon connectivity between brain hemispheres through symmetric adhesion molecule levels and co-fasciculation.

Phosphorylation Determines Whether Neuroligin-3 is at Excitatory or Inhibitory Synapses in Different Regions of the Brain

Rapid modeling of an ultra-rare epilepsy patient variant in mice by in utero prime editing

This paper describes a method to rapidly create mouse models of human epilepsy variants using in utero prime editing, enabling the study of seizures in a clinically relevant timeframe.

Cas9 fusions for precision in vivo editing

The paper develops a high-throughput workflow to assess Cas9 editing precision and identifies a novel Cas9 fusion that improves knockin performance in vivo.

NPRL3: Direct Effects on Human Phenotypic Variability, mTOR Signaling, Subcellular mTOR Localization, Cortical Lamination, and Seizure Susceptibility

The paper investigates NPRL3 variants' effects on mTOR signaling, cortical development, and epilepsy by using CRISPR/Cas9 knockout in cell lines and mice, showing mTOR hyperactivation and cortical dyslamination.