Genome biologyGenomicsPeer-reviewed journal article
Genome-wide CRISPR guide RNA design and specificity analysis with GuideScan2
Henri SchmidtiD, Minsi ZhangiD, Dimitar Chakarov, Vineet BansaliD, Haralambos A. MourelatosiD, Francisco J. Sánchez RiveraiD, Scott W. LoweiD, Andrea VenturaiD, Christina S. LeslieiD, Yuri PritykiniD
Abstract
We present GuideScan2 for memory-efficient, parallelizable construction of high-specificity CRISPR guide RNA (gRNA) databases and user-friendly gRNA/library design in custom genomes. GuideScan2 analysis identified widespread confounding effects of low-specificity gRNAs in published CRISPR knockout, interference and activation screens and enabled construction of a ready-to-use gRNA library that reduced off-target effects in a novel gene essentiality screen. GuideScan2 also enabled the design and experimental validation of allele-specific gRNAs in a hybrid mouse genome.
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- 44
- FWCI
- 8.97
- Field percentile
- top 1%
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