Molecular CellMolecular BiologyPeer-reviewed journal article
Sister chromatid cohesion halts DNA loop expansion
Nathalie BastiéiD, Christophe ChapardiD, Axel CournaciD, Sanae Nejmi, Henri Mboumba, Olivier GadaliD, Agnès ThierryiD, Frédéric BeckouëtiD, Romain KoszuliD
Abstract
Eukaryotic genomes are folded into DNA loops mediated by structural maintenance of chromosomes (SMC) complexes such as cohesin, condensin and Smc5/6. This organization regulates different DNA-related processes along the cell cycle such as transcription, recombination, segregation and DNA repair. During G2/M stages, SMC-mediated DNA loops coexist with cohesin complexes involved in sister chromatid cohesion (SCC). However, the articulation between the establishment of SCC and the formation of SMC-mediated DNA loops along the chromatin remains unknown. Here we show that SCC is indeed a barrier to cohesin-mediated DNA loop expansion in G2.
- Citations
- 28
- FWCI
- 3.53
- Field percentile
- top 5%
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Smc3 acetylation, Pds5 and Scc2 control the translocase activity that establishes cohesin dependent chromatin loops
Nathalie Bastié, Christophe Chapard, … Romain Koszul·Nature Structural & Molecular BiologyPeer-reviewed·Jun 1, 2022
- A major role for Eco1 in regulating cohesin-mediated mitotic chromosome folding
Lise Dauban, Rémi Montagne, … Frédéric Beckouët·bioRxiv·Mar 26, 2019
- Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase
P. Batty, C. C. H. Langer, … D. W. Gerlich·bioRxiv·Jan 12, 2023
- Extensive mutual influences of SMC complexes shape 3D genome folding
Han Zhao, Lirong Shu, … Haoyue Zhang·NaturePeer-reviewed·Feb 26, 2025
- Loop-extruding Smc5/6 organizes transcription-induced positive DNA supercoils
Kristian E. Jeppsson, Biswajit Pradhan, … Camilla Björkegren·Molecular CellPeer-reviewed·Jan 30, 2024
- Scc2-Mediated Loading Of Cohesin Onto Chromosomes In G1 Yeast Cells Is Insufficient To Build Cohesion During S Phase
Kim A Nasmyth·bioRxiv·Apr 3, 2017