bioRxivNeurosciencePreprint
A microRNA atlas of the human prefrontal cortex across the adult lifespan
Irais E. Valenzuela-ArzetaiD, Pourya Naderi YeganehiD, Anupama RaiiD, Jane Banahan, Artemis IatrouiD, Leinal Sejour, Madison M. Uyemura, Isaac H. SolomoniD, Nikolaos P. DaskalakisiD, Ioannis VlachosiD, Winston HideiD, Frank John SlackiD, Maria MavrikakiiD
Abstract
Aging of the human brain is characterized by widespread changes in gene expression regulated in part by microRNAs (miRNAs). We present a lifespan miRNA atlas of the human dorsolateral prefrontal cortex generated from small RNA sequencing of 113 postmortem samples spanning 18 to 100 years of age. Differential expression analysis revealed progressive age-associated remodeling of miRNA expression, with the strongest differences observed between old and young individuals. Among the significantly altered miRNAs, miR-34a-5p emerged as one of the most robustly upregulated miRNAs in the aged cortex, alongside additional aging-associated miRNAs including miR-155-5p, miR-132-3p, miR-212-3p, miR-449a, and members of the miR-302 family. This atlas provides a resource for investigating miRNA dysregulation and small RNA regulatory networks during human cortical aging.
- organism: human
- assay: bulk RNA-seq
- research focus: gene regulatory network inference
- research focus: differential expression
- approach: statistical model
- Citations
- 0
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- A spatio-temporal brain miRNA expression atlas identifies sex-independent age-related microglial driven miR-155-5p increase
Annika Engel, Viktoria Wagner, … Andreas Keller·Nature CommunicationsPeer-reviewed·May 17, 2025
- Identification of Circular RNAs associated with Ageing of the Dorsolateral Prefrontal Cortex across the Adult Lifespan
Fatemeh Amjadi‐Moheb, Sumangali Gobhidharan, … Karen A. Mather·Neurobiology of AgingPeer-reviewed·Oct 9, 2025
- Aging Rhesus Macaque show tissue and sex-specific balance of drifting and coordinated miRNA programs
Shusruto Rishik, Erik Nutma, … Andreas Keller·bioRxiv·May 12, 2026
- Human brain microRNAs exhibit cell-type specificity and agedependence
Serafima Dubnov, Lihen Laski, … Hermona Soreq·bioRxiv·Aug 2, 2026
- Temporal and Spatial Scales of Human Resting-state Cortical Activity Across the Lifespan
John Bero, Colin Humphries, … Daeyeol Lee·Journal of NeurosciencePeer-reviewed·Nov 17, 2025
- A multi-omic atlas of the human frontal cortex for aging and Alzheimer's disease research
P. De Jager, Y. Ma, … D. A. Bennett·bioRxiv·Jan 23, 2018