Mosquito-borne diseases and control

This paper demonstrates that rolling circle amplification (RCA) is a high-fidelity, cost-effective alternative to plasmid preparation for generating infectious viral clones.

This study identifies differential defective RNA (D-RNA) diversity between intracellular and encapsidated alphavirus compartments, showing that some D-RNAs arise de novo and are not packaged, with subgenomic D-RNAs identified as a novel subtype.

The paper traces incursion pathways of Aedes albopictus into Australia using genomics, finding marine routes from East Asia, contrasting with Ae. aegypti's aerial routes from tourism hotspots.

This study examines how temperature affects mosquito gene expression and ZIKV infection outcomes by comparing transcriptomes at different temperatures.

This study identifies fine-scale environmental and demographic factors influencing West Nile virus human illness in Chicago using spatial and statistical modeling.

This study shows that postnatal Zika virus infection in infant macaques leads to long-term behavioral, motor, and cognitive deficits along with structural and functional brain changes.

The paper models dengue transmission from humans to mosquitoes and shows that administering defective virus reduces viral load and inhibits transmission.

The paper genotypes Aedes aegypti mosquitoes captured in Tokyo airports to infer their origins and assess invasion risks.

This paper investigates whether saxitoxin from cyanobacteria worsens neural damage caused by Zika virus in Brazil, finding increased cell death and brain abnormalities in experimental models.

The paper identifies miR-124 as a positive regulator of alphavirus infection through a genome-wide miRNA screen and shows its direct interaction with viral RNA.

The paper uses metagenomic sequencing to identify a yellow fever outbreak in Edo State, Nigeria, from undiagnosed fever cases.

This study characterizes the developmental transcriptome of Aedes albopictus mosquitoes using RNAseq, identifying gene expression patterns and comparing them to Aedes aegypti.

The paper uses genomic data to show multiple ZIKV introductions and cryptic transmission in Colombia, with most transmission occurring locally.

The paper evaluates the effect of defective interfering particles on dengue virus within-host dynamics using population of models to estimate their therapeutic potential.

This study shows that genetic diversity in Collaborative Cross mice affects ZIKV infection outcomes, including viral replication and disease severity, independent of Oas1b.

The paper introduces a high-throughput tool to detect arboviruses in mosquitoes from Zika-endemic regions, showing its effectiveness in identifying circulating viruses.

The paper updates the phylogeny of Chikungunya virus and identifies lineage-specific RNA architecture in its untranslated regions.

The paper identifies novel RNA viruses in Anopheles mosquitoes from Senegal and Cambodia, revealing virome diversity and potential interactions with small RNA pathways.

This paper develops a hybrid ARIMA-NNAR model to forecast dengue cases in three regions, showing improved accuracy over existing methods.

The paper analyzes Musashi binding elements in Zika and related Flavivirus 3’UTRs using a thermodynamic model to show ZIKV MBEs are maximally accessible and linked to neurotropism.

The paper uses comparative genomics to analyze 3’UTR RNA structures in flaviviruses, identifying conserved xrRNAs and duplicated structures across different viral groups.

The paper uses genome-wide SNPs to trace the origin of pyrethroid-resistant Aedes aegypti incursions at international ports, identifying Bali and Pacific Islands as key sources.

The paper identifies domains in the Sindbis virus TF protein that regulate its palmitoylation state and its incorporation into virions.

This study generates Ae. aegypti mosquitoes engineered with synthetic miRNAs to reduce ZIKV infection, dissemination, and transmission.

This study infects olive baboons with Zika virus during mid-gestation and observes fetal cerebral cortical injury and fetal death, providing a new non-human primate model for studying Congenital Zika Syndrome.

This study identifies RNA structural elements in ZIKV strains that influence infectivity through comparative in vivo analysis.

The paper presents an improved Aedes aegypti genome assembly, AaegL5, which enhances biological understanding and vector control efforts by resolving key genomic features and identifying new targets for intervention.

This study uses barcoded Zika virus to track genetic bottlenecks during transmission in vitro and in mosquitoes, showing stochasticity in viral dissemination.

The paper demonstrates unbiased sequencing of a pathogen directly from mosquitoes using handheld nanopore sequencing for field-based biosurveillance.