Zebrafish Biomedical Research Applications

Structural Evolution of Neural Networks during Zebrafish Early Development

The paper uses live-cell imaging to study neural network development in zebrafish, revealing conserved structural dynamics and optimization strategies.

sox17 mutagenesis reveals microglia can be impacted by genetic maternal effects

The paper identifies a genetic maternal effect on microglia development through sox17 mutation in zebrafish, showing that maternal genotype impacts microglia and yolk sac precursor cells.

Collagen4 fluorescent protein knock-in lines reveal remodeling of cardiovascular basement membranes

This study uses fluorescent protein knock-in lines in zebrafish to track Collagen4 remodeling in cardiovascular development and regeneration.

Accelerating drug development in infectious diseases using zebrafish disease models supported by pharmacokinetic pharmacodynamic modeling as new approach methodology (NAM)

Zebrafish larva infection experiments plus pharmacokinetic-pharmacodynamic (PKPD) modelling gave bedaquiline's in vivo Emax and EC50 against M. marinum in about 2 months; waterborne clarithromycin barely entered larvae.

I. Phantom: a multiplex CRISPR-Cas9 gRNA toolkit for efficient generation of fully unpigmented F0 zebrafish

The paper develops a multiplex CRISPR-Cas9 gRNA toolkit to generate fully unpigmented zebrafish larvae with minimal mortality, enabling improved imaging without chemical inhibitors.

Asymmetric regeneration dynamics in zebrafish pectoral fins

This study quantifies spatial asymmetry in zebrafish pectoral fin regeneration, revealing differential gene expression and signaling dynamics between anterior and posterior regions.

Open-source virtual reality platform for head-fixed adult zebrafish

The paper introduces an open-source VR platform for head-fixed adult zebrafish to study behavior and neuronal activity in controlled virtual environments.

gene2fish: a multi-gene spatial expression browser for in situ hybridization data across zebrafish development

gene2fish arranges 53,595 Thisse in situ hybridization images of 8,263 zebrafish genes into a gene-by-stage grid, with anatomy-term search for validating scRNA-seq markers.

Optimization of breeding conditions in Danionella cerebrum to advance its use as a vertebrate model

This study optimizes breeding conditions for Danionella cerebrum to enhance egg production and collection efficiency.

Laminin and Fibronectin contribute to Guided Endothelial Self-Organization during Intersegmental Vessel Formation

This study shows laminin and fibronectin guide endothelial self-organization during intersegmental vessel formation in zebrafish by constraining network-forming behavior.

A motor endurance assay for zebrafish larvae: proof of principle in chemical and genetic models of neurological disease

This paper develops a motor endurance assay for zebrafish larvae to study neurological diseases, finding reduced endurance in TDP43-overexpressing larvae and MPP+-treated larvae.

Cellular and molecular census of the pleiotropic disease locus Negr1 in zebrafish

The paper identifies cell types expressing Negr1 in zebrafish and links its expression to diverse traits, suggesting broad gene action.