Biochemical and Structural Characterization

This paper identifies four novel C-terminal protein-sorting signals and their cognate processing enzymes in prokaryotes using computational analysis.

This paper uses visible light to control specific protein reactions in mammalian cells, revealing early stages of cell adhesion and mechanotransduction.

The paper develops a high-throughput method to characterize protein-protein interactions and design orthogonal coiled-coil pairs, identifying a large set of specific interactions.

The paper introduces helixCAM, a platform for engineering specific cell-cell interactions in bacteria and human cells using coiled-coil peptides, enabling multicellular assembly applications.

The paper introduces InterSpy, a synthetic biology tool that forms membrane-membrane interfaces using SpyTag/SpyCatcher, and demonstrates its function in cell-free systems and cells.

The paper develops a random forest method to identify fibrillar adhesins in bacterial proteomes, discovering over 6,500 candidates and 24 novel adhesive domain families.

The paper studies the role of two conserved histidines in the intein splicing of SufB in Mycobacterium tuberculosis, showing their impact on splicing and cleavage rates.

The paper develops a new chimeric lectin, Janus lectin, with enhanced valency and specificity for fucose and galactose, and demonstrates its application in forming protocell-like structures.

The paper describes a chemical method to design proteasome-like nanoreactors using micelle-assisted labeling and bioconjugation.

The paper expands the SiMPl plasmid toolbox to include spectinomycin/streptomycin resistance by introducing a new plasmid pair and validating a strategy for splitting antibiotic resistance enzymes.

This paper designs stable coiled-coil peptides for cellular regulation and detects SARS-CoV-2 spike protein-mediated cell fusion.

This paper introduces a method to insert synthetic peptides into proteins in eukaryotic cells to enable chemical modifications and non-canonical amino acids, validated using GFP, NaV1.5, and P2X2 receptors.

The paper describes a split-intein system for regulating site-specific recombination using protein trans-splicing, demonstrating its application as a reversible AND gate.

This paper introduces thermomers, temperature-controlled protein dimerization domains that enable remote control of intracellular interactions in various biological contexts.

This paper introduces a genetically encoded adhesion toolbox in E. coli to program multicellular morphologies and patterns through modular cell-cell adhesion.

This paper demonstrates a method for tunable extracellular self-assembly of multi-protein conjugates using Bacillus subtilis, enhancing enzyme resilience and enabling functional multi-protein complex formation.