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The paper designs proteins that bind intrinsically disordered regions with high affinity and specificity across diverse targets.

This study presents an optimized phosphoproteomics workflow using SP3 beads with improved phosphopeptide identification and detection of multiply phosphorylated peptides in HEK-293T cells.

This paper develops a protocol to accurately measure the mass of large DNA plasmids using mass photometry by converting dsDNA to ssDNA-like particles, achieving mass accuracy within 1-3%.

This study investigates the molecular interactions of eIF2β with eIF5, eIF2B, and 5MP1 using crystallography and NMR, revealing binding sites and regulatory roles of CK2 phosphorylation.

The paper experimentally separates the effects of amino acid substitutions on function and abundance for SARS-CoV-2 Mpro, revealing their distinct contributions to DMS outcomes.

This study identifies an oxidase/reductase pair in Kratom that catalyzes the epimerization of 3S to 3R stereocenters in monoterpene indole alkaloids, enabling access to previously inaccessible compounds.

The paper shows that phosphorylation of TEL-ABL makes it resistant to asciminib, but dephosphorylation restores sensitivity.

This study identifies a Cu(II) exchange mechanism between YcnI and YcnJ in B. subtilis, showing their roles in copper acquisition and regulation under copper limitation.

This study identifies RPL7 and DDX21 as co-chaperones that enhance HTLV-1 Gag's ability to anneal tRNAPro to the viral genomic RNA primer binding site.

This study compares human MDH1 and MDH2 isoforms to show structural and enzymatic differences that explain their distinct regulatory roles in metabolism.

This study optimized a colorimetric assay to identify inhibitors of ZIKV RdRp, finding two compounds with inhibitory activity.

This paper shows that RNA chemical probing reagents can react with protein amino acids, altering protein-RNA binding interactions.

This study uses molecular dynamics simulations to analyze the dynamic behavior of S. aureus Type I signal peptidases SpsA and SpsB, including the impact of the P29S mutation on their structure and function.

This study characterizes the conformational changes of the BiP-GRP94 chaperone complex and identifies the structural basis of their interaction without co-chaperones.

This paper introduces an AMPK PHICS platform that enables controlled protein phosphorylation under physiological conditions, demonstrating its utility in regulating oncogenic signaling and phase separation.

This study examines how different heme sources affect gene expression in Trypanosoma cruzi, identifying novel heme-responsive genes and proteins.

The paper designs a panel of selective peroxygenases for terpene oxyfunctionalization using computational methods, resulting in improved enzymatic functions and new products.

The paper describes an automated system for producing secreted proteins in transfected mammalian cells, enabling high-throughput, flexible, and cost-effective protein production for research.