A. Ashare

P. aeruginosa tRNA-fMet halves secreted in outer membrane vesicles suppress lung inflammation in Cystic Fibrosis

This paper shows tobramycin reduces inflammation in cystic fibrosis by increasing 5' tRNA-fMet halves in P. aeruginosa outer membrane vesicles, which suppress IL-8 and neutrophilic inflammation in human cells and mice.

Characterization of SARS-CoV-2 Convalescent Patients' Serological Repertoire Reveals High Prevalence of Iso-RBD Antibodies

This study characterizes the serological repertoire of SARS-CoV-2 convalescent patients, revealing a high prevalence of iso-RBD antibodies that do not recognize prefusion-stabilized S, and shows that vaccination with prefusion-stabilized S reduces their prevalence.

Mrs4 loss of function in fungi during adaptation to the cystic fibrosis lung

The paper identifies MRS4 mutations in fungi associated with cystic fibrosis lung infections, showing they confer a fitness advantage by enhancing iron acquisition in iron-limited environments.

ScRNA-seq Expression of IFI27 and APOC2 Identifies Four Alveolar Macrophage Superclusters in Healthy BALF

The paper identifies four alveolar macrophage superclusters in healthy bronchoalveolar lavage fluid using scRNA-seq and finds they are functionally specialized with conserved frequencies across individuals.

Metabolic basis for the evolution of a common pathogenic Pseudomonas aeruginosa variant

The paper shows that metabolic regulation differences explain the frequent occurrence of lasR loss-of-function mutations in Pseudomonas aeruginosa, linking them to disease severity and evolution.

Tobramycin-induced secretion of P. aeruginosa 5' tRNA-fMet halves suppresses lung inflammation via AGO2 gene silencing

This study shows tobramycin reduces lung inflammation in pwCF by increasing 5' tRNA-fMet halves that suppress IL-8 and neutrophil recruitment via AGO2 gene silencing.

Aspergillus fumigatus In-Host HOG pathway mutation for Cystic Fibrosis Lung Microenvironment Persistence

This paper identifies a unique Pbs2 MAPKK allele in Aspergillus fumigatus isolates from CF patients that enables lung persistence by enhancing osmotic stress response in low oxygen but reducing oxidative stress resistance in standard conditions.

Balancing positive and negative selection: in vivo evolution of Candida lusitaniae MRR1

The paper analyzes MRR1 allele evolution in Candida lusitaniae isolates from a chronic infection, revealing tradeoffs between high Mrr1 activity conferring antifungal resistance and hydrogen peroxide resistance.

Profiling of bacterial and fungal microbial communities in cystic fibrosis sputum using RNA

This paper introduces a method to detect bacterial and fungal taxa in CF sputum using RNA-based NanoString probes, showing taxon-specificity and linear response over three logs of input RNA.