A. Bogdanova

Excess fermentation and lactic acidosis as detrimental functions of the gut microbes in treatment-naive TB patients

This study identifies excess fermentation and lactic acidosis in TB patients' gut microbiomes as detrimental to host immunity and potential treatment targets.

Efficient precision genome editing of Chlamydomonas reinhardtii with CRISPR/Cas

This paper develops an efficient method for scarless genome editing in Chlamydomonas reinhardtii using CRISPR/Cas, enabling precise knock-in mutagenesis without prior mutant lines.

FastCAT accelerates absolute quantification of proteins by using multiple short non-purified chimeric standards

FastCAT improves absolute protein quantification by using chimeric standards, achieving accuracy and efficiency in quantifying neurological markers in human cerebrospinal fluid.

Median based absolute quantification of proteins using Fully Unlabelled Generic Internal Standard

This paper introduces a method for absolute protein quantification using a fully unlabelled generic internal standard, showing improved performance over existing methods.

Recapitulating evolutionary divergence in a single regulatory element causes expression changes of the lens gene Tdrd7

The paper investigates how mutations in a regulatory element upstream of Tdrd7 affect lens development by replacing the mouse sequence with the blind mole rat sequence in mice.

Mitochondrial Na+ controls oxidative phosphorylation and hypoxic redox signalling

This study shows that mitochondrial Na+ acts as a second messenger regulating OXPHOS and ROS production through interactions with phospholipids during hypoxia.

FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing

FlexiBAC simplifies baculovirus production and enables proteolytic maturation of secreted proteins, demonstrating effectiveness in producing active TGF-β family growth factors.

Filament formation by the translation factor eIF2B regulates protein synthesis in starved cells

This study shows that eIF2B filament formation in starved yeast cells regulates translation by acidification-induced assembly, independent of Gcn2 kinase, promoting stress survival and recovery.

MS Western, a method of multiplexed absolute protein quantification is a practical alternative to western blotting

MS Western provides a method for absolute protein quantification without antibodies or labels, outperforming Western blotting in specificity and sensitivity.