C. Engel

Novel fluorescence-based methods to determine infarct and scar size in murine models of reperfused myocardial infarction

The paper compares fluorescence staining methods to classical histochemical techniques for determining infarct and scar size in murine reperfused myocardial infarction models.

Structural basis of archaeal RNA polymerase transcription elongation and Spt4/5 recruitment

This study determines the structure of archaeal RNA polymerase and its interaction with Spt4/5, revealing conformational changes and a DNA-binding role for Spt5.

The human RNA polymerase I structure reveals an HMG-like transcription factor docking domain specific to metazoans

The paper determines the structure of human RNA polymerase I, identifies a novel HMG-like domain in RPA1, and shows its role in recruiting Topoisomerase 2a and UBF for transcription regulation.

Cryo-EM structure of the NLRP3 decamer bound to the cytokine release inhibitory drug CRID3

The paper determines the cryo-EM structure of NLRP3 bound to CRID3, revealing how the inhibitor stabilizes the protein and provides insights for drug development.

Nuclear stabilisation of p53 requires a functional nucleolar surveillance pathway

This study identifies the nucleolar surveillance pathway (NSP) as a central regulator of p53 stability, showing its role in integrating various cellular stresses and its potential as a therapeutic target in cancer.

Structure of human RNA Polymerase III

The paper determines the structure of human RNA Polymerase III using cryo-EM and maps mutations linked to neurodevelopmental disorders and viral hypersensitivity.

RNA polymerase I passage through nucleosomes depends on its lobe binding subunits

This study shows that RNA polymerase I can transcribe through nucleosomes, and its subunits Rpa34.5/Rpa49 and Rpa12.2 are critical for this process.

DNA origami-based single-molecule force spectroscopy unravels the molecular basis of RNA Polymerase III pre-initiation complex stability

The paper uses DNA origami force clamps to study how force affects initiation complex stability in RNA polymerase II and III systems, revealing Bdp1's critical role in RNAP III stability.