H. Urlaub

Structural basis of archaeal RNA polymerase transcription elongation and Spt4/5 recruitment

This study determines the structure of archaeal RNA polymerase and its interaction with Spt4/5, revealing conformational changes and a DNA-binding role for Spt5.

Plk1 inhibition delays mitotic entry revealing prophase-specific changes to the phosphoproteome

This study shows that Plk1 inhibition delays mitotic entry, revealing prophase-specific phosphoproteome changes and highlighting Plk1's role in the prophase to prometaphase transition.

Unbiased complexome profiling and global proteomics analysis reveals mitochondrial impairment and potential changes at the intercalated disk in presymptomatic R14Delta/+ mice hearts

Control of a type III-Dv CRISPR-Cas system by the transcription factor RpaB and interaction of its leader transcript with the DEAD-box RNA helicase CrhR

This study identifies RpaB as a light-and redox-responsive transcription factor regulating a type III-Dv CRISPR-Cas system in Synechocystis sp. PCC 6803 and shows CrhR interacts with the leader transcript posttranscriptionally.

Phosphoproteomic identification of Mos-MAPK targets in meiotic cell cycle and asymmetric oocyte divisions

The paper identifies Mos-MAPK targets in starfish oocytes using phosphoproteomics, revealing roles in mRNA polyadenylation and cytoskeletal regulation during meiosis.

The previously uncharacterized RnpM (YlxR) protein modulates the activity of ribonuclease P in Bacillus subtilis

The paper identifies RnpM (YlxR) as a novel modulator of RNase P activity in B. subtilis, reducing its ability to process pre-tRNAs.

Phosphorylation-dependent interactions of VAPB and ELYS contribute to the temporal progression of mitosis

This study identifies phosphorylation-dependent interactions between VAPB and ELYS that facilitate nuclear envelope formation during mitosis.

Phosphatases modified by LH signaling in ovarian follicles: testing their role in regulating the NPR2 guanylyl cyclase

The paper identifies phosphatases rapidly modified by luteinizing hormone in ovarian follicles, revealing their role in regulating NPR2 guanylyl cyclase dephosphorylation.

Uncovering the temporal dynamics and regulatory networks of thermal stress response in a hyperthermophile using transcriptomics and proteomics

This study uses transcriptomics and proteomics to analyze the thermal stress response in Pyrococcus furiosus, revealing rapid transcriptome reprogramming and persistent protein upregulation during heat shock.

Functional genomics identifies extension of complex N-glycans as a mechanism to evade lysis by natural killer cells

The paper identifies SPPL3 as a gene that enables tumor cells to evade NK cell lysis by altering N-glycan structures, and shows that inhibiting N-glycan maturation restores NK sensitivity.

Human TRMT2A methylates tRNA and contributes to translation fidelity

This study identifies TRMT2A as a tRNA methyltransferase that contributes to translation fidelity by modifying tRNA with m5U at position 54.

Metamorphic proteins at the basis of human autophagy initiation and lipid transfer

This study identifies the molecular mechanism of autophagy initiation by revealing the role of metamorphic proteins ATG13-101 in forming the autophagy initiation supercomplex.

Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA

The paper identifies Npl3's role in mRNP assembly by recruiting components to the transcription site and transferring them onto mRNA.

Hsp multichaperone complex buffers pathologically modified Tau

This study identifies the Hsp70/Hsp90 multichaperone complex as a critical regulator of Tau by stabilizing it and preventing aggregation, with p23 enhancing complex formation and CHIP facilitating degradation.

Progressive protein aggregation in PRPF31 patient retinal pigment epithelium cells: the mechanism and its reversal through activation of autophagy

This study shows that PRPF31 mutations cause progressive protein aggregation in RPE cells, which can be reversed by autophagy activation.

Structure of the human FERRY Rab5 effector complex

The paper determines the cryo-EM structure of the human FERRY Rab5 effector complex and shows Fy-2 acts as a binding hub for subunits and mRNA/Rab5.

A viral ADP-ribosyltransferase attaches RNA chains to host proteins

This study identifies a viral ADP-ribosyltransferase, ModB, that attaches RNA chains to host proteins through an 'RNAylation' reaction, revealing a novel role for NAD-RNA in modulating host translation.

Evaluation and Optimization of High-Field Asymmetric Waveform Ion Mobility Spectrometry for Multiplexed Quantitative Site-specific N-glycoproteomics

This study optimizes FAIMS settings for improved identification and quantification of site-specific N-glycopeptides using TMT labeling and Glyco-SPS-MS3 methods.

SANS (USH1G) regulates pre-mRNA splicing by mediating the intra-nuclear transfer of tri-snRNP complexes

This study identifies SANS as a regulator of pre-mRNA splicing by facilitating the transfer of tri-snRNP complexes between Cajal bodies and nuclear speckles, linking it to Usher syndrome pathophysiology.

Itk promotes the integration of TCR and CD28 costimulation, through its direct substrates, SLP-76 and Gads

This study identifies Itk-mediated phosphorylation sites on SLP-76 and Gads that regulate TCR/CD28 costimulation-induced transcriptional responses.

Dipeptidyl peptidase 9 triggers BRCA2 degradation by the N-degron pathway to promote DNA-damage repair

This study identifies DPP9 as a regulator of BRCA2 stability and DNA-damage repair by promoting its degradation via the N-degron pathway.

Post-Translational Modifications Soften Vimentin Intermediate Filaments

This study investigates how post-translational modifications, specifically phosphorylation and 14-3-3 binding, affect the mechanical properties of vimentin intermediate filaments.

SugarQuant: a streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics

SugarQuant provides a pipeline for site-specific N-glycoproteomics, enabling confident quantification of glycoforms in complex samples.