Exploiting O-GlcNAc Transferase promiscuity to dissect site-specific O-GlcNAcylation
C. W. Mitchell, S. G. Bartual, A. T. Ferenbach, C. Scavenius, D. M. F. van Aalten
This study develops a method to produce site-specific, homogeneous mono-glycosylated proteins for studying O-GlcNAcylation using OGT's tolerance for cysteine and co-expressed OGA.
bioRxiv · Jul 31, 2023Biochemistry